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Cationic triple-chain amphiphiles facilitate vesicle fusion compared to double-chain or single-chain analogues
- Source :
- Biochimica et biophysica acta. 1564(2)
- Publication Year :
- 2002
-
Abstract
- Cationic, triple-chain amphiphiles promote vesicle fusion more than structurally related double-chain or single-chain analogues. Two types of vesicle fusion experiments were conducted, mixing of oppositely charged vesicles and acid-triggered self-fusion of vesicles composed of cationic amphiphile and anionic cholesteryl hemisuccinate (CHEMS). Vesicle fusion was monitored by standard fluorescence assays for intermembrane lipid mixing, aqueous contents mixing and leakage. Differential scanning calorimetry was used to show that triple-chain amphiphiles lower the lamellar–inverse hexagonal (Lα–HII) phase transition temperature for dipalmitoleoylphosphatidylethanolamine. The triple-chain amphiphiles may enhance vesicle fusion because they can stabilize the inversely curved membrane surfaces of the fusion intermediates, however, other factors such as extended conformation, packing defects, chain motion, or surface dehydration may also contribute. From the perspective of drug delivery, the results suggest that vesicles containing cationic, triple-chain amphiphiles (and cationic, cone-shaped amphiphiles in general) may be effective as fusogenic delivery capsules.
- Subjects :
- Vesicle fusion
Lipid Bilayers
Biophysics
Molecular Conformation
Phosphatidic Acids
Membrane curvature
Calorimetry
Biochemistry
Membrane Fusion
Drug Delivery Systems
Cations
Amphiphile
Organic chemistry
Cationic liposome
Lipid bilayer
Fluorescent Dyes
Calorimetry, Differential Scanning
Chemistry
Rhodamines
Vesicle
Phosphatidylethanolamines
Cationic polymerization
Gene Transfer Techniques
Temperature
Lipid bilayer fusion
Cell Biology
Hydrogen-Ion Concentration
Quaternary Ammonium Compounds
4-Chloro-7-nitrobenzofurazan
Drug delivery
Phosphatidylcholines
Molecular shape
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 1564
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....26ac8ae9e73a422cdf0d1586af920aab