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Clinical Capillary Zone Electrophoresis of Serum Proteins: Balancing High Sensitivity and High Specificity
- Source :
- Clinical Chemistry. 49:1419-1421
- Publication Year :
- 2003
- Publisher :
- Oxford University Press (OUP), 2003.
-
Abstract
- Capillary zone electrophoresis (CZE) is documented to have high sensitivity and specificity for the detection of monoclonal (M) proteins. In two large (>1500 participants) prospective studies in which CZE was compared with “immunofixation/immunoelectrophoresis”, the sensitivity of CZE was 95% (1)(2). This is superior to the sensitivity of agarose gel electrophoresis (AGE), which Katzmann et al. (1) found to be 91% and Meunier (3) found to be 92.2% (evaluation of 2060 sera containing an M-protein). Especially in the β-globulin region, CZE with Paragon CZE 2000 (Beckman-Coulter) is superior to AGE for detection of low-concentration M-proteins (IgA and light chains) (3)(4). The specificity of CZE is reported to be high (98.6%) and comparable to that of AGE (1)(5). In a prospective study (2), we described three M-proteins with high pI values that migrated in the slow γ region on AGE but were not detected with Paragon. In addition, one high-concentration IgG|gl with a pI ≈7 that migrated in the mid-γ region on AGE was not separated by CZE. The Paragon has recently been upgraded [modified buffer, higher voltage (10.3 kV), more efficient cooling, adapted software (1.6.02)], and according … [↵][1]bAuthor for correspondence. [1]: #xref-corresp-2-1
- Subjects :
- Immunofixation
Chromatography
medicine.diagnostic_test
biology
Chemistry
Biochemistry (medical)
Clinical Biochemistry
Electrophoresis, Capillary
Immunoelectrophoresis
Sensitivity and Specificity
Molecular biology
Blood proteins
Myeloma Proteins
Capillary electrophoresis
Agarose gel electrophoresis
medicine
biology.protein
Humans
Prospective Studies
Subjects
Details
- ISSN :
- 15308561 and 00099147
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Clinical Chemistry
- Accession number :
- edsair.doi.dedup.....26a6515f76bec045fc2e76140d12f705
- Full Text :
- https://doi.org/10.1373/49.8.1419