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Digitoxin-like immunoreactivity in sera of mice after feeding with chinese medicine Chan Su: study of protein binding of Chan Su in normal sera, uremic sera and sera from patients with liver disease
- Source :
- Clinica chimica acta; international journal of clinical chemistry. 305(1-2)
- Publication Year :
- 2001
-
Abstract
- Traditional Chinese medicines are readily available without prescription from herbal drug stores. One such Chinese medicine, Chan Su, which is prepared from the skin gland of toads, has cardiotonic effect due to bufadienolides. Here we report rapid detection of the presence of Chan Su in blood using the fluorescence polarization immunoassay for digitoxin. In our study mice were fed with a dose of 75 mg/kg of Chan Su and blood was drawn before, and 1 and 2 h after feeding. We observed significant digitoxin-like immunoreactivity in the sera. For example in one mouse the digitoxin-like immunoreactivity was undetectable before feeding with Chan Su, but was 19.7 ng/ml 1 h and 8.8 ng/ml 2 h afterwards. The apparent half-life of Chan Su is approximately 1 h in mice. In another experiment, we studied protein binding of Chan Su by measuring total and free Chan Su concentrations (ultrafiltrate prepared by using Centrifree Micropartition Filter, molecular weight cutoff: 30000 Da). Chan Su was strongly bound to serum proteins. We observed higher free fraction in uremic sera and sera from patients with liver disease. We identified albumin as one of the proteins that bind Chan Su in serum.
- Subjects :
- medicine.medical_specialty
Digitoxin
Clinical Biochemistry
Bufadienolide
Pharmacognosy
Biochemistry
Liver disease
chemistry.chemical_compound
Mice
Internal medicine
medicine
Animals
Uremia
business.industry
Liver Diseases
Biochemistry (medical)
Albumin
General Medicine
medicine.disease
Blood proteins
Bufanolides
Endocrinology
chemistry
Free fraction
business
medicine.drug
Subjects
Details
- ISSN :
- 00098981
- Volume :
- 305
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Accession number :
- edsair.doi.dedup.....26a42e0bc71f3eaca7f79e847fad81b9