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Reaching for far-flung antigen: How solid-core podosomes of dendritic cells transform into protrusive structures
- Source :
- Communicative & Integrative Biology, Communicative & Integrative Biology, 7, 1-5, Communicative & Integrative Biology, 7, 5, pp. 1-5
- Publication Year :
- 2014
- Publisher :
- Informa UK Limited, 2014.
-
Abstract
- We recently identified a novel role for podosomes in antigen sampling. Podosomes are dynamic cellular structures that consist of point-like concentrations of actin surrounded by integrins and adaptor proteins such as vinculin and talin. Podosomes establish cellular contact with the extracellular matrix (ECM) and facilitate cell migration via ECM degradation. In our recent paper, we studied podosomes of human dendritic cells (DCs), major antigen presenting cells (APC) that take-up, process, and present foreign antigen to naive T-cells. We employed gelatin-impregnated porous polycarbonate filters to demonstrate that the mechanosensitive podosomes of DCs selectively localize to regions of low-physical resistance such as the filter pores. After degradation of the gelatin, podosomes increasingly protrude into the lumen of these pores. These protrusive podosome-derived structures contain several endocytic and early endosomal markers such as clathrin, Rab5, and VAMP3, and, surprisingly, also contain C-type lectins, a type of pathogen recognition receptors (PRRs). Finally, we performed functional uptake experiments to demonstrate that these PRRs facilitate uptake of antigen from the opposite side of the filter. Our data provide mechanistic insight in how dendritic cells sample for antigen across epithelial barriers for instance from the lumen of the lung and gut.
- Subjects :
- Pattern recognition receptors
biology
Podosome
Endosome
Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]
Short Communication
Integrin
Invadopodia
Leukocyte extravasation
Membrane trafficking
Vinculin
Clathrin
Cell biology
Extracellular matrix
Antigen sampling
biology.protein
C-type lectins
General Agricultural and Biological Sciences
Antigen-presenting cell
GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
Receptor-mediated endocytosis
Dendritic cell
Actin
Subjects
Details
- ISSN :
- 19420889
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Communicative & Integrative Biology
- Accession number :
- edsair.doi.dedup.....26a42db5a5509d7a7a86d1f143da7f75