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Gut microbial profile analysis by MiSeq sequencing of pancreatic carcinoma patients in China
- Source :
- Oncotarget
- Publication Year :
- 2017
- Publisher :
- Impact Journals, LLC, 2017.
-
Abstract
- Pancreatic carcinoma (PC) is a lethal cancer. Gut microbiota is associated with some risk factors of PC, e.g. obesity and types II diabetes. However, the specific gut microbial profile in clinical PC in China has never been reported. This prospective study collected 85 PC and 57 matched healthy controls (HC) to analyze microbial characteristics by MiSeq sequencing. The results showed that gut microbial diversity was decreased in PC with an unique microbial profile, which partly attributed to its decrease of alpha diversity. Microbial alterations in PC featured by the increase of certain pathogens and lipopolysaccharides-producing bacteria, and the decrease of probiotics and butyrate-producing bacteria. Microbial community in obstruction cases was separated from the un-obstructed cases. Streptococcus was associated with the bile. Furthermore, 23 microbial functions e.g. Leucine and LPS biosynthesis were enriched, while 13 functions were reduced in PC. Importantly, based on 40 genera associated with PC, microbial markers achieves a high classification power with AUC of 0.842. In conclusion, gut microbial profile was unique in PC, providing a microbial marker for non-invasive PC diagnosis.
- Subjects :
- 0301 basic medicine
MiSeq sequencing
Gut flora
medicine.disease_cause
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Medicine
pancreatic carcinoma
gut microbiota
biology
Traditional medicine
business.industry
Streptococcus
biomarkers
Cancer
alpha diversity
biology.organism_classification
medicine.disease
Obesity
030104 developmental biology
Oncology
chemistry
Microbial population biology
Leucine
business
Bacteria
Research Paper
Subjects
Details
- ISSN :
- 19492553
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....26916b5f863e0b6aa5584f224a5905c5