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Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response
- Source :
- Clinical proteomics
- Publisher :
- Springer Nature
-
Abstract
- Background: Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. Results: A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. Conclusions: Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies.
- Subjects :
- Fibrinogen-gamma chain
Proteomics
Pathology
medicine.medical_specialty
medicine.medical_treatment
Clinical Biochemistry
030232 urology & nephrology
chemistry.chemical_element
Calcium
Pharmacology
Peritoneal dialysis
03 medical and health sciences
0302 clinical medicine
medicine
Molecular Biology
030304 developmental biology
Calcium metabolism
0303 health sciences
business.industry
Research
Albumin
General Medicine
medicine.disease
3. Good health
chemistry
Peritoneal dialysis effluent
Proteome
Molecular Medicine
2D-Gel Electrophoresis
Protein identification
business
Kidney disease
Subjects
Details
- Language :
- English
- ISSN :
- 15590275
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Clinical Proteomics
- Accession number :
- edsair.doi.dedup.....2688621b8ddfe039e045ea50a334dd84
- Full Text :
- https://doi.org/10.1186/1559-0275-11-17