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Structural characterization of sulfated arabinans extracted fromCladophora glomerataKützing and their macrophage activation
- Source :
- Bioscience, Biotechnology, and Biochemistry. 80:972-982
- Publication Year :
- 2016
- Publisher :
- Informa UK Limited, 2016.
-
Abstract
- Water-soluble sulfated heteropolysaccharides were extracted from Cladophora glomerata Kützing and fractionated by ion-exchange chromatography, which yielded two subfractions, F1 and F2. The crude and fractionated polysaccharides (F1 and F2) mostly consisted of carbohydrates (62.8–74.5%) with various amounts of proteins (9.00–17.3%) and sulfates (16.5–23.5%), including different levels of arabinose (41.7–54.4%), galactose (13.5–39.0%), glucose (0.80–10.6%), xylose (6.84–13.4%), and rhamnose (0.20–2.83%). Based on the size exclusion chromatography (SEC) profiles, the crude and fractions mainly contained one peak with shoulders having molecular weight (Mw) ranges of 358–1,501 × 103. The F1 fraction stimulated RAW264.7 cells to produce considerable amounts of nitric oxide and cytokines compared to the crude and F2 fraction. The backbone of the most potent immunostimulating fraction (F1) was α-(1→4)-L-arabinopyranoside with galactose and xylose residues as branches at O-2 position, and sulfates mainly at O-2 position as well.
- Subjects :
- 0301 basic medicine
Arabinose
Rhamnose
Size-exclusion chromatography
Fraction (chemistry)
Xylose
Biology
Nitric Oxide
Polysaccharide
01 natural sciences
Applied Microbiology and Biotechnology
Biochemistry
Cell Line
Analytical Chemistry
Mice
03 medical and health sciences
chemistry.chemical_compound
Sulfation
Adjuvants, Immunologic
Chlorophyta
Polysaccharides
Animals
Molecular Biology
chemistry.chemical_classification
Chromatography
Plant Extracts
Sulfates
010405 organic chemistry
Macrophages
Organic Chemistry
Galactose
Water
General Medicine
Macrophage Activation
0104 chemical sciences
Molecular Weight
Glucose
030104 developmental biology
Carbohydrate Sequence
Solubility
chemistry
Cytokines
Biotechnology
Subjects
Details
- ISSN :
- 13476947 and 09168451
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....265af248fb9052ebf2cdcaefc272f04a