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The HMG box-containing nucleolar transcription factor UBF interacts with a specific subunit of RNA polymerase I
- Source :
- The EMBO Journal. 13:190-199
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- The mammalian transcription activator protein UBF contains five tandemly repeated HMG homology domains which are required for DNA binding. We have used highly purified RNA polymerase I (Pol I) and upstream binding factor (UBF) and investigated whether these two proteins interact in solution. We show by a variety of different experimental approaches, such as immunoprecipitation, glycerol gradient sedimentation, affinity chromatography and protein blotting, that UBF physically associates with Pol I. Mutational analysis reveals that the HMG boxes play an important role in this specific interaction. UBF binds to mouse and yeast Pol I, demonstrating that the interaction of UBF with Pol I has been conserved during evolution. Interestingly, in both species one Pol I-specific subunit (34.5 kDa in yeast and 62 kDa in mouse) was recognized by UBF. No specific interaction was observed with Pol II. Unexpectedly, UBF was found to associate also with a unique subunit of yeast Pol III. This apparent specific interaction of UBF with the two classes of RNA polymerases may reflect functionally important interactions of HMG box-containing transcription factors with the transcriptional apparatus.
- Subjects :
- Molecular Sequence Data
RNA polymerase II
Saccharomyces cerevisiae
Biology
DNA-binding protein
Chromatography, Affinity
General Biochemistry, Genetics and Molecular Biology
RNA polymerase III
Mice
RNA Polymerase I
Transcription (biology)
RNA polymerase I
Animals
Molecular Biology
Transcription factor
Polymerase
Base Sequence
General Immunology and Microbiology
General Neuroscience
High Mobility Group Proteins
Biological Evolution
Precipitin Tests
Molecular biology
Cell biology
DNA-Binding Proteins
Oligodeoxyribonucleotides
Mutation
Transcription preinitiation complex
biology.protein
Pol1 Transcription Initiation Complex Proteins
Protein Binding
Transcription Factors
Research Article
Subjects
Details
- ISSN :
- 02614189
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....265833c92ea9a8ff786010b02fccd90b