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CpG-C ODN M362 as an immunoadjuvant for HBV therapeutic vaccine reverses the systemic tolerance against HBV
- Source :
- International Journal of Biological Sciences
- Publication Year :
- 2022
- Publisher :
- Ivyspring International Publisher, 2022.
-
Abstract
- Chronic Hepatitis B virus (CHB) infection is a global public health problem. Oligodeoxynucleotides (ODNs) containing class C unmethylated cytosine-guanine dinucleotide (CpG-C) motifs may provide potential adjuvants for the immunotherapeutic strategy against CHB, since CpG-C ODNs stimulate both B cell and dendritic cell (DC) activation. However, the efficacy of CpG-C ODN as an anti-HBV vaccine adjuvant remains unclear. In this study, we demonstrated that CpG M362 (CpG-C ODN) as an adjuvant in anti-HBV vaccine (cHBV-vaccine) successfully and safely eliminated the virus in HBV-carrier mice. The cHBV-vaccine enhanced DC maturation both in vivo and in vitro, overcame immune tolerance, and recovered exhausted T cells in HBV-carrier mice. Furthermore, the cHBV-vaccine elicited robust hepatic HBV-specific CD8+ and CD4+ T cell responses, with increased cellular proliferation and IFN-γ secretion. Additionally, the cHBV-vaccine invoked a long-lasting follicular CXCR5+ CD8+ T cell response following HBV re-challenge. Taken together, CpG M362 in combination with rHBVvac cleared persistent HBV and achieved long-term virological control, making it a promising candidate for treating CHB.
- Subjects :
- Male
Applied Microbiology and Biotechnology
Immunoadjuvant
Mice
Hepatitis B, Chronic
adjuvant
Adjuvants, Immunologic
Animals
Medicine
chronic hepatitis B
Hepatitis B Vaccines
Molecular Biology
Ecology, Evolution, Behavior and Systematics
CXCR5+ CD8+ T cells
CpG-C ODN
business.industry
Cell Biology
Mice, Inbred C57BL
Disease Models, Animal
Oligodeoxyribonucleotides
CpG site
Immunology
Therapeutic vaccine
therapeutic vaccine
business
Dinucleoside Phosphates
Research Paper
immune-tolerance
Developmental Biology
Subjects
Details
- ISSN :
- 14492288
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....264c4e251de56d131be39f424c8b7053
- Full Text :
- https://doi.org/10.7150/ijbs.62424