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Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients
- Source :
- Nature Communications, Vol 11, Iss 1, Pp 1-7 (2020), Nature Communications
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Given the ongoing SARS-CoV-2 pandemic, identification of immunogenic targets against the coronavirus spike glycoprotein will provide crucial advances towards the development of sensitive diagnostic tools and potential vaccine candidate targets. In this study, using pools of overlapping linear B-cell peptides, we report two IgG immunodominant regions on SARS-CoV-2 spike glycoprotein that are recognised by sera from COVID-19 convalescent patients. Notably, one is specific to SARS-CoV-2, which is located in close proximity to the receptor binding domain. The other region, which is localised at the fusion peptide, could potentially function as a pan-SARS target. Functionally, antibody depletion assays demonstrate that antibodies targeting these immunodominant regions significantly alter virus neutralisation capacities. Taken together, identification and validation of these neutralising B-cell epitopes will provide insights towards the design of diagnostics and vaccine candidates against this high priority coronavirus.<br />Characterisation of the human antibody response to SARS-CoV-2 can help the design of serological tests and vaccines. Here, the authors identify two linear epitopes in SARS-CoV-2 spike protein that elicit neutralising antibodies in several patients and could thus be useful for serology and vaccine development.
- Subjects :
- 0301 basic medicine
Science
Pneumonia, Viral
General Physics and Astronomy
Antibodies, Viral
medicine.disease_cause
Article
Antibodies
General Biochemistry, Genetics and Molecular Biology
Epitope
Neutralization
Virus
Betacoronavirus
03 medical and health sciences
0302 clinical medicine
medicine
Humans
Amino Acid Sequence
skin and connective tissue diseases
lcsh:Science
Pandemics
Peptide sequence
Coronavirus
chemistry.chemical_classification
Multidisciplinary
biology
Immunodominant Epitopes
SARS-CoV-2
fungi
COVID-19
General Chemistry
biology.organism_classification
Antibodies, Neutralizing
Virology
body regions
030104 developmental biology
chemistry
Viral infection
Immunoglobulin G
030220 oncology & carcinogenesis
Spike Glycoprotein, Coronavirus
biology.protein
Epitopes, B-Lymphocyte
lcsh:Q
Antibody
Coronavirus Infections
Glycoprotein
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....2640c34238c15442b36b3df7dbec3883