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Effects of gliadin stimulation on bone marrow-derived dendritic cells from HLA-DQ8 transgenic MICE
- Source :
- Digestive and Liver Disease 40 (2008): 927–935., info:cnr-pdr/source/autori:Ciccocioppo R.; Rossi M.; Pesce I.; Ricci G.; Millimaggi D.; Maurano F.; Corazza G.R./titolo:Effects of gliadin stimulation on bone marrow-derived dendritic cells from HLA-DQ8 transgenic MICE/doi:/rivista:Digestive and Liver Disease/anno:2008/pagina_da:927/pagina_a:935/intervallo_pagine:927–935/volume:40
- Publication Year :
- 2008
- Publisher :
- Editrice Gastroenterologica Italiana., Ospedaletto, Pisa , Italia, 2008.
-
Abstract
- BACKGROUND AND AIM: Gliadin presentation by HLA-DQ2/8 molecules to T cells plays a crucial role in triggering the inflammatory cascade in coeliac disease. We aimed to study the immunological effects of gliadin stimulation on dendritic cells (DCs) from HLA-DQ8 transgenic and BALB/c mice. METHODS: Bone marrow-derived DCs were stimulated with alpha-chymotrypsin-digested gliadin or ovoalbumin (100mug/ml). Modification of DC maturation, through HLA-DQ8 and MHC class II expression, and activation, by CD80 and CD86, was assessed by flow cytometry. The ability of pulsed and unpulsed DCs to prime T cells was evaluated by mixed leucocyte reaction. The expression of interleukin-4, -10, -12p70 and interferon-alpha, as well as of Toll-like receptor-4, -7, -8, -9 was determined by ELISA and real-time RT-PCR, respectively. RESULTS: Gliadin stimulation induced DC maturation (p
- Subjects :
- CD4-Positive T-Lymphocytes
Cells
Receptor expression
Bone Marrow Cells
Mice, Transgenic
Major histocompatibility complex
Animals
Celiac Disease
Cell Differentiation
Cells, Cultured
Dendritic Cells
Gliadin
HLA-DQ Antigens
Interferon-alpha
Interleukins
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred BALB C
Toll-Like Receptors
Transgenic
Coeliac disease
Flow cytometry
medicine
Lymphocyte Culture Test
Inbred BALB C
CD86
MHC class II
Cultured
Hepatology
biology
medicine.diagnostic_test
Gastroenterology
nutritional and metabolic diseases
medicine.disease
Molecular biology
Mixed
Immunology
biology.protein
CD80
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Digestive and Liver Disease 40 (2008): 927–935., info:cnr-pdr/source/autori:Ciccocioppo R.; Rossi M.; Pesce I.; Ricci G.; Millimaggi D.; Maurano F.; Corazza G.R./titolo:Effects of gliadin stimulation on bone marrow-derived dendritic cells from HLA-DQ8 transgenic MICE/doi:/rivista:Digestive and Liver Disease/anno:2008/pagina_da:927/pagina_a:935/intervallo_pagine:927–935/volume:40
- Accession number :
- edsair.doi.dedup.....263b697b3ce0dd88cc6fd8d7130b137f