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Evaluation of stool antigen test, PCR on ORAL samples and serology for the noninvasive detection of Helicobacter pylori infection in children
- Publication Year :
- 2004
- Publisher :
- BLACKWELL PUBLISHING INC, 2004.
-
Abstract
- Background. Endoscopy represents the gold standard for the diagnosis of Helicobacter pylori infection. We evaluated three noninvasive tests in a group of children: the immunoassay for detection of H. pylori stool antigen, the polimerase chain reaction for identification of bacterial DNA on the oral cavity and the serum specific antibodies. Materials and Methods. One hundred and ninety children underwent endoscopy for various gastrointestinal symptoms. H. pylori stool antigen and anti-H. pylori antibodies were assayed by commercial kits. The bacterial DNA on saliva and oral plaque was detected by a seminested PCR. Results. Based on the positivity of culture or urease rapid test and histology, infection was detected in 47 patients. The statistical analysis showed that, for the detection of the infection, stool antigen assay is more effective in sensitivity and negative predictive value (91.5% and 96.5%), whereas specificity and positive predictive values appear slightly better in serology (89.6% and 76.0%). Correlations between serum IgG both with patients’ age (r = 0.21, p
- Subjects :
- DNA, Bacterial
Male
Saliva
Adolescent
Biopsy
serology
Polymerase Chain Reaction
Sensitivity and Specificity
Helicobacter Infections
law.invention
Serology
Feces
Antigen
children
law
Gastroscopy
Humans
Medicine
oral pcr
stool test
Child
Polymerase chain reaction
Antigens, Bacterial
Helicobacter pylori
medicine.diagnostic_test
biology
business.industry
Stool test
Gastroenterology
Infant
General Medicine
Gold standard (test)
Antibodies, Bacterial
Urease
Infectious Diseases
Gastric Mucosa
Child, Preschool
Immunoglobulin G
Immunoassay
Immunology
biology.protein
Female
Reagent Kits, Diagnostic
Antibody
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....2630a947500e460090d68c4986d6da51