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Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
- Source :
- PLoS ONE, Vol 17, Iss 1, p e0261252 (2022), Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP, PLoS ONE, Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- Made available in DSpace on 2022-04-29T08:46:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Background Fluoride has become widely used in dentistry because of its effectiveness in caries control. However, evidence indicates that excessive intake interferes with the metabolic processes of different tissues. Thus, this study aimed to investigate the effects of long-term exposure to F on the parotid salivary gland of mice, from the analysis of oxidative, proteomic and genotoxic parameters. Materials and methods The animals received deionized water containing 0, 10 or 50 mg/L of F, as sodium fluoride, for 60 days. After, parotid glands were collected for analysis of oxidative biochemistry, global proteomic profile, genotoxicity assessment and histopathological analyses. Results The results revealed that exposure to fluoride interfered in the biochemical homeostasis of the parotid gland, with increased levels of thiobarbituric acid reactive species and reduced glutathione in the exposed groups; as well as promoted alteration of the glandular proteomic profile in these groups, especially in structural proteins and proteins related to oxidative stress. However, genotoxic assessment demonstrated that exposure to fluoride did not interfere with DNA integrity in these concentrations and durations of exposure. Also, it was not observed histopathological alterations in parotid gland. Conclusions Thus, our results suggest that long-term exposure to fluoride promoted modulation of the proteomic and biochemical profile in the parotid glands, without inducing damage to the genetic component. These findings reinforce the importance of rationalizing the use of fluorides to maximize their preventative effects while minimizing the environmental risks. Laboratory of Functional and Structural Biology Institute of Biological Sciences Federal University of Para, PA Laboratory of Clinical Immunology and Oxidative Stress Pharmacy Faculty Institute of Health Science Federal University of Para, PA Evandro Chagas Institute, PA Department of Biological Sciences Bauru Dental School University of São Paulo, SP Department of Chemistry University of Nebraska-Lincoln Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP
- Subjects :
- Proteomics
Male
Time Factors
Proteome
Science
Biochemistry
Thiobarbituric Acid Reactive Substances
Salivary Glands
Antioxidants
Fluorides
Mice
Exocrine Glands
stomatognathic system
DNA-binding proteins
Medicine and Health Sciences
Animals
Parotid Gland
Parotid Glands
Multidisciplinary
Chemical Compounds
Biology and Life Sciences
Proteins
Cell Biology
Glutathione
STAT proteins
Chemistry
Oxidative Stress
Gene Expression Regulation
Physical Sciences
Sodium Fluoride
Medicine
CAMUNDONGOS
Lipid Peroxidation
Anatomy
Digestive System
Oxidation-Reduction
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....262c9f9e940aeca21834eb608df9dcb0