Back to Search
Start Over
Effect of treatment with the antioxidant alpha-lipoic (thioctic) acid on heart and kidney microvasculature in spontaneously hypertensive rats
- Source :
- Clinical and experimental hypertension (New York, N.Y. : 1993). 38(1)
- Publication Year :
- 2015
-
Abstract
- Endothelial cells represent an important vascular site of signaling and development of damage during ischemia, inflammation and other pathological conditions. Excessive reactive oxygen species production causes pathological activation of endothelium including exposure of cell to adhesion molecules. Intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM-1) are members of the immunoglobulin super-family which are present on the surface of endothelial cells. These molecules represent important markers of endothelial inflammation. The present study was designed to investigate, with immunochemical and immunohistochemical techniques, the effect of treatment with (+/-)-alpha lipoic (thioctic) acid and its enantiomers on heart and kidney endothelium in spontaneously hypertensive rats (SHR). Arterial hypertension is accompanied by an increased oxidative stress status in the heart characterized by thiobarbituric acid reactive substances (TBARS) and nucleic acid oxidation increase. The higher oxidative stress also modifies adhesion molecules expression. In the heart VCAM-1, which was higher than ICAM-1 and PECAM-1, was increased in SHR. ICAM-1, VCAM-1 and PECAM-1 expression was significantly greater in the renal endothelium of SHR. (+/-)-Alpha lipoic acid and (+)-alpha lipoic acid treatment significantly decreased TBARS levels, the nucleic acid oxidation and prevented adhesion molecules expression in cardiac and renal vascular endothelium. These data suggest that endothelial molecules may be used for studying the mechanisms of vascular injury on target organs of hypertension. The effects observed after treatment with (+)-alpha lipoic acid could open new perspectives for countering heart and kidney microvascular injury which represent a common feature in hypertensive end-organs damage.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Endothelium
Physiology
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1
030204 cardiovascular system & hematology
medicine.disease_cause
Kidney
Thiobarbituric Acid Reactive Substances
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Rats, Inbred SHR
Internal Medicine
medicine
TBARS
Animals
Alpha-lipoic acid
endothelial adhesion molecules
spontaneously hypertensive rats
Endothelial Cells
Myocardium
Rats
Reactive Oxygen Species
Thioctic Acid
Treatment Outcome
Coronary Vessels
Endothelium, Vascular
Hypertension
Microvessels
Cell adhesion
Cell adhesion molecule
Soluble cell adhesion molecules
General Medicine
Lipoic acid
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Biochemistry
chemistry
cardiovascular system
Oxidative stress
Subjects
Details
- ISSN :
- 15256006
- Volume :
- 38
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Clinical and experimental hypertension (New York, N.Y. : 1993)
- Accession number :
- edsair.doi.dedup.....260dd85e50aa95dea5381be365f1e899