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Regulatory Effect of Cannabidiol (CBD) on Decreased β-Catenin Expression in Alopecia Models by Testosterone and PMA Treatment in Dermal Papilla Cells
- Source :
- Journal of Pharmacopuncture, Journal of Pharmacopuncture, Vol 24, Iss 2, Pp 68-75 (2021)
- Publication Year :
- 2021
-
Abstract
- Objectives: The hair follicle is composed of more than 20 kinds of cells, and mesoderm derived dermal papilla cells and keratinocytes cooperatively contribute hair growth via Wnt/β-catenin signaling pathway. We are to investigate β-catenin expression and regulatory mechanism by CBD in alopecia hair tissues and dermal papilla cells. Methods : : We performed structural and anatomical analyses on alopecia patients derived hair tissues using microscopes. Pharmacological effect of CBD was evaluated by β-catenin expression using RT-PCR and immunostaining experiment. Results : : Morphological deformation and loss of cell numbers in hair shaft were observed in alopecia hair tissues. IHC experiment showed that loss of β-catenin expression was shown in inner shaft of the alopecia hair tissues, indicating that β-catenin expression is a key regulatory function during alopecia progression. Consistently, β-catenin expression was decreased in testosterone or PMA treated dermal papilla cells, suggesting that those treatments are referred as a model on molecular mechanism of alopecia using dermal papilla cells. RT-PCR and immunostaining experiments showed that β-catenin expression was decreased in RNA level, as well as decreased β-catenin protein might be resulted from ubiquitination. However, CBD treatment has no changes in gene expression including β-catenin, but the decreased β-catenin expression by testosterone or PMA was restored by CBD pretreatment, suggesting that potential regulatory effect on alopecia induction of testosterone and PMA. Conclusion : : CBD might have a modulating function on alopecia caused by hormonal or excess of signaling pathway, and be a promising application for on alopecia treatment.
- Subjects :
- medicine.medical_specialty
RM1-950
phorbol 12-myristate 13-acetate (PMA)
dermal papilla
Internal medicine
Gene expression
medicine
Miscellaneous systems and treatments
Testosterone
Pharmacology
integumentary system
Chemistry
RZ409.7-999
Wnt signaling pathway
Hair follicle
alopecia
cannabidiol (CBD)
Endocrinology
medicine.anatomical_structure
Dermal papillae
Complementary and alternative medicine
Catenin
testosterone
Medicine
Original Article
Therapeutics. Pharmacology
Signal transduction
Immunostaining
wnt/β-catenin
Subjects
Details
- ISSN :
- 20936966
- Volume :
- 24
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of pharmacopuncture
- Accession number :
- edsair.doi.dedup.....25fd5bbcf3a59e632bf06ab2fbde33b1