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Studies on gene control regions. 1. Chemical synthesis of lactose operator deoxyribonucleic acid segments
- Source :
- Biochemistry. 16:1765-1772
- Publication Year :
- 1977
- Publisher :
- American Chemical Society (ACS), 1977.
-
Abstract
- The chemical synthesis of lactose operator DNA segments is described. The 31-base-paired duplex contains the DNA recognized by lac repressor protein and twofold rotationally symmetric base pairs on either side of the tight binding region. The synthesis includes the deoxyoligonucleotides d(T-G-T-G-G), d(A-A-T-T-G-T-G-A-G), d(C-G-G-A-T-A-A-C-A-A-T-T), d(T-C-A-C-A), d(T-G-T-G-A-A-A-T-T-G-T), d(T-A-T-C-C-G-C-T-C-A-C), and d(A-A-T-T-C-C-A-C-A). These deoxyoligonucleotides were characterized by two-dimensional sequencing techniques, paper chromatography, and thin-layer chromatography.
Details
- ISSN :
- 15204995 and 00062960
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....25f5abf3201751b340f48b0fc131e662