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Prevalence of Iron Transport Gene on Pathogenicity-Associated Island of Uropathogenic Escherichia coli in E. coli O157:H7 Containing Shiga Toxin Gene
- Source :
- Journal of Clinical Microbiology. 39:2300-2305
- Publication Year :
- 2001
- Publisher :
- American Society for Microbiology, 2001.
-
Abstract
- Uropathogenc Escherichia coli (UPEC) CFT073 has a pathogenicity-associated island (PAI CFT073 ), which causes pyelonephritis and cystitis. Using PCR method, we found the prrA gene of PAI CFT073 in E. coli O157:H7 EDL933. Further detailed PCR screening of 38 open reading frames, the right and left junction sequences of PAI CFT073 , revealed that it is the prrA-modD-yc73-fepC gene cluster but not the PAI CFT073 present in E. coli O157:H7 EDL933. A rapid preliminary analysis suggested that the prrA-modD-yc73-fepC gene cluster of the PAI CFT073 , is present in 43 strains of E. coli O157:H7 containing Shiga toxin (Stx) gene but absent in 19 strains of E. coli O157:H7 without Stx gene. A strict co-occurrence of the prrA-modD-yc73-fepC gene cluster and Stx genes was observed, regardless of their origin. The prrA-modD-yc73-fepC gene cluster encode proteins probably involved in iron uptake system, which strongly suggests the importance of iron metabolism in the Stx-mediated virulence. In addition, the prrA-modD-yc73-fepC gene cluster may be used as a diagnostic marker to distinguish E. coli O157:H7 strains containing Stx gene from that without Stx gene, and possibly to quickly detect other pathogenic gram-negative bacteria containing the Stx gene.
- Subjects :
- Microbiology (medical)
Iron
Virulence
Escherichia coli O157
Shiga Toxins
medicine.disease_cause
Polymerase Chain Reaction
Microbiology
Cystitis
Gene cluster
medicine
Animals
Humans
Gene
Escherichia coli
Genetics
Pyelonephritis
biology
Nucleic Acid Hybridization
Bacteriology
Shiga toxin
biology.organism_classification
Enterobacteriaceae
Open reading frame
Genes, Bacterial
Multigene Family
biology.protein
Bacteria
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....25df39dfbc20cb8427d2a10c085a61eb