Additional file 1 of PDCD6 cooperates with C-Raf to facilitate colorectal cancer progression via Raf/MEK/ERK activation

Additional file 1: Figure S1. The PDCD6 expression in different patient samples from the GEPIA database of different kinds of diseases. Figure S2a. PDCD6-KD and c-Raf-KD inhibited the MAPK pathway. Figure S2b. Combined downregulation of PDCD6 with oxiliplatin effect on cell proliferation in HCT116 and HCT15. Figure S2c. Cell proliferation assays on RAF709 and Trametinib treatment. Figure S2d. Colony formation assays on RAF709 and Trametinib treatment. Figure S3a. Representative immunohistochemistry images of the expression of p-c-Raf, p-MEK, c-Caspase 3 and p-Stat 3 in xenograft tumors from nude mice subcutaneously injected with HCT116 cells. *, P < 0.05. Figure S3b. HE staining showed the influences of PDCD6-KD and PDCD6-OE on mice tumor samples. Table S1. Characteristics of 93 patients with colorectal cancer included in the study. Table S2. For the knockdown of human PDCD6-specific shRNAs. Table S3. For the knockdown of human PDCD6-siRNA and c-Raf-siRNA. Table S4. Oligonucleotide sequences used in reverse-transcription PCR and real-time PCR.