Supplementary Methods, Figures 1 - 11 from Chromosomal Instability Affects the Tumorigenicity of Glioblastoma Tumor-Initiating Cells

Supplementary Figure 1 shows Nestin expression. Supplementary Figure 2 shows Sox2 expression. Supplementary Figure 3 shows differentiation of GNS cells. Supplementary Figure 4 shows checkpoint assay and FISH data. Supplementary Figure 5 shows karyotypes of GliNS2 cells. Supplementary Figure 6 shows p53 and p21 response. Supplementary Figure 7 shows FISH and karyotypes of PDX lines.Supplementary Figure 8 shows Sox2 and Nestin expression in stable GFP expressing lines. Supplementary Figure 9 shows limiting dilution assay in vitro. Supplementary Figure 10 shows FACS profiles for growth assay. Supplementary Figure 11 shows astrocyte differentiation of GFP expressing lines.