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Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
- Source :
- Digibug. Repositorio Institucional de la Universidad de Granada, instname, Polymers, Digibug: Repositorio Institucional de la Universidad de Granada, Universidad de Granada (UGR), Polymers, Vol 13, Iss 1568, p 1568 (2021), Volume 13, Issue 10
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tightjunction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use.<br />CTS-115 Tissue Engineering Group and by the Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministry of Science and Innovation, Instituto de Salud Carlos III, grant FIS PI21/0981 (cofinanced by FEDER funds, European Union).
- Subjects :
- Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation [Medical Subject Headings]
Chemicals and Drugs::Biomedical and Dental Materials::Biocompatible Materials [Medical Subject Headings]
Stromal cell
Polymers and Plastics
Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Uroplakins::Uroplakin II [Medical Subject Headings]
Organic chemistry
Células madre mesenquimatosas
Biofabrication
Chemicals and Drugs::Carbohydrates::Polysaccharides::Sepharose [Medical Subject Headings]
Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Cytoskeletal Proteins::Plakins::Desmoplakins [Medical Subject Headings]
Membrana mucosa
Article
Glycosaminoglycan
human Wharton jelly mesenchymal stromal cells
03 medical and health sciences
0302 clinical medicine
QD241-441
Stroma
Keratin
Wharton's jelly
Human Wharton jelly mesenchymal stromal cells
030304 developmental biology
Urothelial mucosa
chemistry.chemical_classification
0303 health sciences
Ensayo de materiales
Anatomy::Urogenital System::Urinary Tract::Ureter [Medical Subject Headings]
biofabrication
Mesenchymal stem cell
General Chemistry
Anatomy::Tissues::Membranes::Mucous Membrane [Medical Subject Headings]
Cell biology
Anatomy::Tissues::Connective Tissue::Wharton Jelly [Medical Subject Headings]
chemistry
030220 oncology & carcinogenesis
urothelial mucosa
Ex vivo
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Digibug. Repositorio Institucional de la Universidad de Granada, instname, Polymers, Digibug: Repositorio Institucional de la Universidad de Granada, Universidad de Granada (UGR), Polymers, Vol 13, Iss 1568, p 1568 (2021), Volume 13, Issue 10
- Accession number :
- edsair.doi.dedup.....25a36906f46f4ca9e714f5323ded1f0d