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Determination of 2-fluoro-2-deoxy-d-glucose uptake and ATP level for evaluating drug effects in neoplastic cells
- Source :
- Research in Experimental Medicine. 191:27-35
- Publication Year :
- 1991
- Publisher :
- Springer Science and Business Media LLC, 1991.
-
Abstract
- The glucose analogue 2-fluoro-2-deoxy-D-glucose (FDG) was used to study chemosensitivity of two human ovarian cancer cell lines and of murine L1210 cells. Cell viability was determined by measuring intracellular adenosine triphosphate (ATP) with a bioluminescence method, which has been shown to correlate closely with trypan blue, stem cell, and [3H]TdR assays. All three cell lines were sensitive to cytostatic drugs, which exerted a parallel decrease in the intracellular FDG and ATP levels. The two measures correlated positively (r = 0.66, P less than 0.001), indicating that FDG uptake is closely linked with ATP production. Relatively low hexokinase (HK)-to-glucose 6-phosphatase (HK/G6-Pase) ratios were measured, which suggests that the metabolic trapping of FDG 6-phosphate within the cytosol is incomplete. Apparently, these cell lines may not depend exclusively on glycolysis for their energy requirement. We conclude that cell killing caused by cytostatic drugs is associated with a decreased ATP content and FDG uptake. This indicates that not only ATP but also FDG may be used to study drug effects in vitro.
- Subjects :
- Cell Survival
Antineoplastic Agents
Deoxyglucose
Biology
Mice
chemistry.chemical_compound
Adenosine Triphosphate
Fluorodeoxyglucose F18
Tumor Cells, Cultured
Animals
Humans
Glycolysis
Viability assay
Leukemia L1210
Ovarian Neoplasms
Hexokinase
General Medicine
Cytosol
Cell killing
chemistry
Biochemistry
Luminescent Measurements
Female
Drug Screening Assays, Antitumor
Energy Metabolism
2-Deoxy-D-glucose
Adenosine triphosphate
Intracellular
Subjects
Details
- ISSN :
- 14338580 and 03009130
- Volume :
- 191
- Database :
- OpenAIRE
- Journal :
- Research in Experimental Medicine
- Accession number :
- edsair.doi.dedup.....25a2b87c6721ad41810fd144facb69f8