Back to Search
Start Over
Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 2, p e0171192 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science, 2017.
-
Abstract
- Electrochemically reduced water (ERW) is produced near a cathode during electrolysis and exhibits an alkaline pH, contains richly dissolved hydrogen, and contains a small amount of platinum nanoparticles. ERW has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that hydrogen-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of hydrogen levels; however, there is no report verifying the role of dissolved hydrogen in ERW. In this report, we clarify whether the responsive factor for antioxidative activity in ERW is dissolved hydrogen. The intracellular ROS scavenging activity of ERW and hydrogen-dissolved water was tested by both fluorescent stain method and immuno spin trapping assay. We confirm that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity, and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved hydrogen, but lost its activity when autoclaved. An oxygen radical absorbance capacity assay, the 2,2-diphenyl-1-picrylhydrazyl assay and chemiluminescence assay could not detect radical-scavenging activity in both ERW and hydrogen-dissolved water. These results indicate that ERW contains electrolysis-dependent hydrogen and an additional antioxidative factor predicted to be platinum nanoparticles.
- Subjects :
- 0301 basic medicine
Oxygen radical absorbance capacity
Hydrogen
lcsh:Medicine
Platinum nanoparticles
Oxygen
law.invention
chemistry.chemical_compound
0302 clinical medicine
law
Electrochemistry
Nanotechnology
Hydrates
lcsh:Science
Hydrogen peroxide
Cells, Cultured
chemistry.chemical_classification
Multidisciplinary
Oxides
Free Radical Scavengers
Peroxides
Chemistry
Biochemistry
030220 oncology & carcinogenesis
Physical Sciences
Cell lines
Engineering and Technology
Biological cultures
HT1080 cells
Oxidation-Reduction
Research Article
Chemical Elements
chemistry.chemical_element
Electrolysis
03 medical and health sciences
Humans
Platinum
Reactive oxygen species
Spin trapping
lcsh:R
Chemical Compounds
Water
Hydrogen Peroxide
Research and analysis methods
Oxidative Stress
030104 developmental biology
chemistry
Nanoparticles
lcsh:Q
Reactive Oxygen Species
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....259f38b1908d55ce7e493d33d13ffd6b