Back to Search
Start Over
Characterization of resident lymphocytes in human pancreatic islets
- Source :
- Clinical and Experimental Immunology. 187:418-427
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Summary The current view of type 1 diabetes (T1D) is that it is an immune-mediated disease where lymphocytes infiltrate the pancreatic islets, promote killing of beta cells and cause overt diabetes. Although tissue resident immune cells have been demonstrated in several organs, the composition of lymphocytes in human healthy pancreatic islets have been scarcely studied. Here we aimed to investigate the phenotype of immune cells associated with human islets of non-diabetic organ donors. A flow cytometry analysis of isolated islets from perfused pancreases (n = 38) was employed to identify alpha, beta, T, natural killer (NK) and B cells. Moreover, the expression of insulin and glucagon transcripts was evaluated by RNA sequencing. Up to 80% of the lymphocytes were CD3+ T cells with a remarkable bias towards CD8+ cells. Central memory and effector memory phenotypes dominated within the CD8+ and CD4+ T cells and most CD8+ T cells were positive for CD69 and up to 50–70% for CD103, both markers of resident memory cells. The frequency of B and NK cells was low in most islet preparations (12 and 3% of CD45+ cells, respectively), and the frequency of alpha and beta cells varied between donors and correlated clearly with insulin and glucagon mRNA expression. In conclusion, we demonstrated the predominance of canonical tissue resident memory CD8+ T cells associated with human islets. We believe that these results are important to understand more clearly the immunobiology of human islets and the disease-related phenotypes observed in diabetes.
- Subjects :
- CD4-Positive T-Lymphocytes
Male
0301 basic medicine
endocrine system
medicine.medical_specialty
Immunology
CD8-Positive T-Lymphocytes
Biology
Islets of Langerhans
03 medical and health sciences
Interleukin 21
Immune system
Insulin-Secreting Cells
Internal medicine
medicine
Humans
Insulin
Immunology and Allergy
IL-2 receptor
B-Lymphocytes
Pancreatic islets
Original Articles
Middle Aged
Natural killer T cell
Killer Cells, Natural
Diabetes Mellitus, Type 1
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Interleukin 12
Female
Pancreas
CD8
Subjects
Details
- ISSN :
- 13652249 and 00099104
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Immunology
- Accession number :
- edsair.doi.dedup.....2596f1a89500d786a9a51c3483ba0db9
- Full Text :
- https://doi.org/10.1111/cei.12892