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Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis
Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis
- Source :
- Advanced Healthcare Materials. 4:577-584
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- Severe burn injury results in substantial skin loss and cannot be treated by autografts. The Integra Dermal Regeneration Template (IDRT) is the leading synthetic skin substitute because it allows for wound bed regeneration and wound healing. However, all substitutes suffer from slow blood vessel ingrowth and would benefit considerably from enhanced vascularization to nurture tissue repair. It is shown here that by incorporating the human elastic protein tropoelastin into a dermal regeneration template (TDRT) we can promote angiogenesis in wound healing. In small and large animal models comprising mice and pigs, the hybrid TDRT biomaterial and IDRT show similar contraction to autografts and decrease wound contraction compared to open wounds. In mice, TDRT accelerates early stage angiogenesis by 2 weeks, as evidenced by increased angiogenesis fluorescent radiant efficiency in live animal imaging and the expression of endothelial cell adhesion marker CD146. In the pig, a full thickness wound repair model confirms increased numbers of blood vessels in the regenerating areas of the dermis closest to the hypodermis and immediately below the epidermis at 2 weeks post-surgery. It is concluded that including tropoelastin in a dermal regeneration template has the potential to promote wound repair through enhanced vascularization.
- Subjects :
- medicine.medical_specialty
Materials science
Angiogenesis
Biopsy
Sus scrofa
Biomedical Engineering
Neovascularization, Physiologic
Pharmaceutical Science
Artificial skin
Biomaterials
Mice
Dermis
Tropoelastin
medicine
Animals
Humans
Regeneration
Cell Proliferation
Wound Healing
integumentary system
biology
Skin Transplantation
Fibroblasts
Elasticity
Surgery
Cell biology
Endothelial stem cell
Disease Models, Animal
medicine.anatomical_structure
biology.protein
Blood Vessels
CD146
Stress, Mechanical
Wound healing
Blood vessel
Subjects
Details
- ISSN :
- 21922640
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Advanced Healthcare Materials
- Accession number :
- edsair.doi.dedup.....258ef547fd23783c91d872041c36f3fb
- Full Text :
- https://doi.org/10.1002/adhm.201400571