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Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis

Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis

Authors :
Robert Daniels
Roy M. Kimble
Peter K.M. Maitz
Zhe Li
Ali Fathi
Cassandra Chong
Xue-Qing Wang
Yiwei Wang
Suzanne M. Mithieux
Fariba Dehghani
Eleni Panas
John E. Kemnitzer
Anthony S. Weiss
Yvonne Kong
Source :
Advanced Healthcare Materials. 4:577-584
Publication Year :
2014
Publisher :
Wiley, 2014.

Abstract

Severe burn injury results in substantial skin loss and cannot be treated by autografts. The Integra Dermal Regeneration Template (IDRT) is the leading synthetic skin substitute because it allows for wound bed regeneration and wound healing. However, all substitutes suffer from slow blood vessel ingrowth and would benefit considerably from enhanced vascularization to nurture tissue repair. It is shown here that by incorporating the human elastic protein tropoelastin into a dermal regeneration template (TDRT) we can promote angiogenesis in wound healing. In small and large animal models comprising mice and pigs, the hybrid TDRT biomaterial and IDRT show similar contraction to autografts and decrease wound contraction compared to open wounds. In mice, TDRT accelerates early stage angiogenesis by 2 weeks, as evidenced by increased angiogenesis fluorescent radiant efficiency in live animal imaging and the expression of endothelial cell adhesion marker CD146. In the pig, a full thickness wound repair model confirms increased numbers of blood vessels in the regenerating areas of the dermis closest to the hypodermis and immediately below the epidermis at 2 weeks post-surgery. It is concluded that including tropoelastin in a dermal regeneration template has the potential to promote wound repair through enhanced vascularization.

Details

ISSN :
21922640
Volume :
4
Database :
OpenAIRE
Journal :
Advanced Healthcare Materials
Accession number :
edsair.doi.dedup.....258ef547fd23783c91d872041c36f3fb
Full Text :
https://doi.org/10.1002/adhm.201400571