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The Second Receptor Binding Site of the Globular Head of the Newcastle Disease Virus Hemagglutinin-Neuraminidase Activates the Stalk of Multiple Paramyxovirus Receptor Binding Proteins To Trigger Fusion
- Source :
- Journal of Virology. 86:5730-5741
- Publication Year :
- 2012
- Publisher :
- American Society for Microbiology, 2012.
-
Abstract
- The hemagglutinin-neuraminidase (HN) protein of paramyxoviruses carries out three distinct activities contributing to the ability of HN to promote viral fusion and entry: receptor binding, receptor cleavage (neuraminidase), and activation of the fusion protein. The relationship between receptor binding and fusion triggering functions of HN are not fully understood. For Newcastle disease virus (NDV), one bifunctional site (site I) on HN′s globular head can mediate both receptor binding and neuraminidase activities, and a second site (site II) in the globular head is also capable of mediating receptor binding. The receptor analog, zanamivir, blocks receptor binding and cleavage activities of NDV HN′s site I while activating receptor binding by site II. Comparison of chimeric proteins in which the globular head of NDV HN is connected to the stalk region of either human parainfluenza virus type 3 (HPIV3) or Nipah virus receptor binding proteins indicates that receptor binding to NDV HN site II not only can activate its own fusion (F) protein but can also activate the heterotypic fusion proteins. We suggest a general model for paramyxovirus fusion activation in which receptor engagement at site II plays an active role in F activation.
- Subjects :
- viruses
Immunology
Newcastle disease virus
Microbiology
Cell Line
Viral Proteins
Protein structure
Virology
Receptors, Viru
Humans
Viral Protein
5-HT5A receptor
HN Protein
Binding site
Receptor
Viral Fusion Protein
Binding Sites
Paramyxoviridae Infections
biology
Newcastle disease viru
Binding Site
Virus Internalization
Paramyxoviridae Infection
Fusion protein
Molecular biology
Virus-Cell Interactions
Protein Structure, Tertiary
Insect Science
biology.protein
Receptors, Virus
Paramyxovirinae
Carrier Proteins
Carrier Protein
Viral Fusion Proteins
Neuraminidase
Hemagglutinin-neuraminidase
Human
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....258e6cbc7a3ff810e4f45c2241992941