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ELOVL2 promotes cancer progression by inhibiting cell apoptosis in renal cell carcinoma
- Source :
- Oncology Reports
- Publication Year :
- 2021
- Publisher :
- Spandidos Publications, 2021.
-
Abstract
- Renal cell carcinoma (RCC) is an aggressive genitourinary malignancy which has been associated with a poor prognosis, particularly in patients with metastasis, its major subtypes being clear cell RCC (ccRCC), papillary PCC (pRCC) and chromophobe RCC (chRCC). The presence of intracellular lipid droplets (LDs) is considered to be a hallmark of ccRCC. The importance of an altered lipid metabolism in ccRCC has been widely recognized. The elongation of very-long-chain fatty acid (ELOVL) catalyzes the elongation of fatty acids (FAs), modulating lipid composition, and is required for normal bodily functions. However, the involvement of elongases in RCC remains unclear. In the present study, the expression of ELOVL2 in ccRCC was examined; in particular, high levels of seven ELOVL isozymes were observed in primary tumors. Of note, elevated ELOVL2 expression levels were observed in ccRCC, as well as in pRCC and chRCC. Furthermore, a higher level of ELOVL2 was significantly associated with the increased incidence of a poor prognosis of patients with ccRCC and pRCC. The CRISPR/Cas9-mediated knockdown of ELOVL2 resulted in the suppression of the elongation of long-chain polyunsaturated FAs and increased LD production in renal cancer cells. Moreover, ELOVL2 ablation resulted in the suppression of cellular proliferation via the induction of apoptosis in vitro and the attenuation of tumor growth in vivo. On the whole, the present study provides new insight into the tumor proliferation mechanisms involving lipid metabolism, and suggests that ELOVL2 may be an attractive novel target for RCC therapy.
- Subjects :
- renal cell carcinoma
Cancer Research
Fatty Acid Elongases
lipid droplet
Chromophobe cell
Metastasis
Renal cell carcinoma
Cell Line, Tumor
Lipid droplet
medicine
Humans
Carcinoma, Renal Cell
Cell Proliferation
cellular proliferation
business.industry
apoptosis
Cancer
Lipid metabolism
Articles
General Medicine
Cell cycle
Lipid Metabolism
medicine.disease
Kidney Neoplasms
Oncology
Gene Knockdown Techniques
Cancer cell
Disease Progression
Cancer research
fatty acid
CRISPR-Cas Systems
business
elongation of very-long-chain fatty acid 2
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....258c9a8449f4951b246449502b0a4496