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Label-free optical imaging of membrane potential
- Source :
- Curr Opin Biomed Eng
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Offering high temporal resolution, voltage imaging is an important and essential technique in neuroscience. Among different optical imaging approaches, the label-free approach remains attractive due to its unique value coming from free of exogenous chromophores. The intrinsic voltage-indicating signals arising from membrane deformation, membrane spectral change, phase shift, light scattering, and membrane hydration haven been reported. First demonstrated 70 years ago, label-free optical imaging of membrane potential is still at an early stage and the field is challenged by the relatively small signals generated by the intrinsic optical properties. We review major contrast mechanisms used for label-free voltage imaging and discuss several recent exciting advances that could potentially enable membrane potential imaging in mammalian neurons at high speed and high sensitivity.
- Subjects :
- Membrane potential
0303 health sciences
Birefringence
Materials science
business.industry
Biomedical Engineering
Medicine (miscellaneous)
Second-harmonic generation
Bioengineering
02 engineering and technology
021001 nanoscience & nanotechnology
Article
Light scattering
Biomaterials
03 medical and health sciences
Optical imaging
Membrane
Optoelectronics
0210 nano-technology
business
030304 developmental biology
Voltage
Label free
Subjects
Details
- ISSN :
- 24684511
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Current Opinion in Biomedical Engineering
- Accession number :
- edsair.doi.dedup.....256ca20cadf67e00650d5a9ec3cf0452