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Effects of ischemia on intracellular sodium and phosphates in the in vivo rat liver
- Source :
- Journal of applied physiology (Bethesda, Md. : 1985). 81(3)
- Publication Year :
- 1996
-
Abstract
- Metabolic factors that influence the transition form reversible to irreversible ischemic injury were studied in the rat liver in vivo with 31P-nuclear magnetic resonance (NMR) spectroscopy. Hepatic ischemia for 15, 35, or 65 min was produced by occlusion of the hepatic artery and portal vein in rats. Ischemia caused a rapid decrease in the ATP concentration ([ATP])-to-P(i) concentration ratio and pH within 5 min, but there was little change in these variables detectable by 31P-NMR with longer periods of ischemia. After reperfusion, the [ATP] and P(i) concentration returned toward normal values in livers exposed to 15 or 35 min of ischemia, but 65 min of ischemia were associated with only modest recovery in [ATP], and the [ATP] later decreased. Because the 31P-NMR spectrum was similar after brief compared with prolonged ischemia, it appears that neither ATP depletion, P(i) accumulation, nor acidosis predicts metabolic recovery. Hepatic intracellular NA+ was also measured in separate groups of animals by 23Na-NMR in the presence of a shift agent, thulium (III) 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis (methylene-phosphonate) (TmDOTP5-), and by atomic absorption spectroscopy. Under baseline conditions, the concentration of intracellular Na+ was 15.2 mM by atomic absorption spectroscopy and 16.5 mM by 23Na-NMR. Although the 31P-NMR spectrum responded very rapidly to the onset of ischemia, intracellular Na+ concentration measured by 23Na-NMR increased gradually but steadily at approximately 1.0 mM/min during early (up to 15 min) ischemia. These observations demonstrate that a rise in intracellular Na+ does occur early ischemia, that TmDOTP5- can be applied in vivo for analysis of intracellular Na+ in the ischemic liver, and that 31P-NMR spectroscopy is very sensitive to early ischemic injury.
- Subjects :
- Male
medicine.medical_specialty
Magnetic Resonance Spectroscopy
Bioenergetics
Physiology
Sodium
Ischemia
chemistry.chemical_element
Phosphates
Rats, Sprague-Dawley
chemistry.chemical_compound
Adenosine Triphosphate
In vivo
Physiology (medical)
Internal medicine
medicine
Animals
Acidosis
Metabolism
Hydrogen-Ion Concentration
medicine.disease
Rats
Endocrinology
Biochemistry
chemistry
Liver
medicine.symptom
Adenosine triphosphate
Intracellular
Subjects
Details
- ISSN :
- 87507587
- Volume :
- 81
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of applied physiology (Bethesda, Md. : 1985)
- Accession number :
- edsair.doi.dedup.....255546a84131d79a67e2c0e0bff07e3a