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Effective reduction of non-specific binding of blood cells in a microfluidic chip for isolation of rare cancer cells
- Source :
- Biomaterials Science. 6:2871-2880
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry (RSC), 2018.
-
Abstract
- The high purity of target cells enriched from blood samples plays an important role in the clinical detection of diseases. However, non-specific binding of blood cells in the isolated cell samples can complicate downstream molecular and genetic analysis. In this work, we report a simple solution to non-specific binding of blood cells by modifying the surface of microchips with a multilayer nanofilm, with the outmost layer containing both PEG brushes for reducing blood cell adhesion and antibodies for enriching target cells. This layer-by-layer (LbL) polysaccharide nanofilm was modified with neutravindin and then conjugated with a mixture of biotinylated PEG molecules and biotinylated antibodies. Using EpCAM-expressing and HER2-expressing cancer cells in blood as model platforms, we were able to dramatically reduce the non-specific binding of blood cells to approximately 1 cell per mm2 without sacrificing the high capture efficiency of the microchip. To support the rational extension of this approach to other applications for cell isolation and blood cell resistance, we conducted extensive characterization on the nanofilm formation and degradation, antifouling with PEG brushes and introducing functional antibodies. This simple, yet effective, approach can be applied to a variety of microchip applications that require high purity of sample cells containing minimal contamination from blood cells.
- Subjects :
- 0301 basic medicine
Receptor, ErbB-2
Surface Properties
Cell
Biomedical Engineering
Biosensing Techniques
Cell Separation
02 engineering and technology
Antibodies
Polyethylene Glycols
Blood cell
03 medical and health sciences
Cell Line, Tumor
Lab-On-A-Chip Devices
Neoplasms
PEG ratio
Cell Adhesion
medicine
Humans
General Materials Science
Cell adhesion
Blood Cells
biology
Chemistry
Epithelial Cell Adhesion Molecule
Neoplastic Cells, Circulating
021001 nanoscience & nanotechnology
Nanostructures
030104 developmental biology
medicine.anatomical_structure
Cell culture
Biotinylation
Cancer cell
Biophysics
biology.protein
Antibody
0210 nano-technology
Subjects
Details
- ISSN :
- 20474849 and 20474830
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Biomaterials Science
- Accession number :
- edsair.doi.dedup.....253fa567c4f29d32267c1e6f6b4349d3
- Full Text :
- https://doi.org/10.1039/c8bm00864g