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COP1 promotes ABA‐induced stomatal closure by modulating the abundance of ABI/HAB and AHG3 phosphatases
- Source :
- The New Phytologist
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Summary Plant stomata play a crucial role in leaf function, controlling water transpiration in response to environmental stresses and modulating the gas exchange necessary for photosynthesis. The phytohormone abscisic acid (ABA) promotes stomatal closure and inhibits light‐induced stomatal opening. The Arabidopsis thaliana E3 ubiquitin ligase COP1 functions in ABA‐mediated stomatal closure. However, the underlying molecular mechanisms are still not fully understood.Yeast two‐hybrid assays were used to identify ABA signaling components that interact with COP1, and biochemical, molecular and genetic studies were carried out to elucidate the regulatory role of COP1 in ABA signaling.The cop1 mutants are hyposensitive to ABA‐triggered stomatal closure under light and dark conditions. COP1 interacts with and ubiquitinates the Arabidopsis clade A type 2C phosphatases (PP2Cs) ABI/HAB group and AHG3, thus triggering their degradation. Abscisic acid enhances the COP1‐mediated degradation of these PP2Cs. Mutations in ABI1 and AHG3 partly rescue the cop1 stomatal phenotype and the phosphorylation level of OST1, a crucial SnRK2‐type kinase in ABA signaling.Our data indicate that COP1 is part of a novel signaling pathway promoting ABA‐mediated stomatal closure by regulating the stability of a subset of the Clade A PP2Cs. These findings provide novel insights into the interplay between ABA and the light signaling component in the modulation of stomatal movement.
- Subjects :
- 0106 biological sciences
0301 basic medicine
clade A PP2C
Physiology
Ubiquitin-Protein Ligases
Plant Science
ubiquitination
01 natural sciences
Coat Protein Complex I
03 medical and health sciences
chemistry.chemical_compound
Arabidopsis
Phosphoprotein Phosphatases
Arabidopsis thaliana
Abscisic acid
Transpiration
Full Paper
biology
Arabidopsis Proteins
Chemistry
Research
fungi
COP1
food and beverages
Full Papers
biology.organism_classification
ABI1
Ubiquitin ligase
Cell biology
030104 developmental biology
Mutation
Plant Stomata
biology.protein
abscisic acid (ABA)
Phosphorylation
stomatal movement
Signal transduction
Protein Kinases
Abscisic Acid
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14698137 and 0028646X
- Volume :
- 229
- Database :
- OpenAIRE
- Journal :
- New Phytologist
- Accession number :
- edsair.doi.dedup.....25315ab9d88894ef0a63a7f04fa25d8c