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Cirsium japonicum extract induces apoptosis and anti-proliferation in the human breast cancer cell line MCF-7
- Source :
- Molecular medicine reports. 3(3)
- Publication Year :
- 2011
-
Abstract
- Cirsium japonicum is a wild perennial herb that has been used as an anti-hemorrhagic, anti-hypertensive and uretic agent in traditional Chinese medicine. Recently, it was reported that C. japonicum inhibits the growth of implanted cancer cells. However, the molecular mechanisms underlying the anti-cancer properties of C. japonicum are not fully understood. In this study, we investigated the effect of a methanol extract of C. japonicum on cell growth in the human breast cancer cell line MCF-7. C. japonicum extract inhibited the cell proliferation of MCF-7 cells in a time- and dose-dependent manner, as evaluated by the MTT assay. Furthermore, C. japonicum extract induced an anti-proliferative effect by causing G1 phase cell cycle arrest and also induced apoptosis by affecting mitochondrial apoptotic events, as determined by nuclear derangement, flow cytometry and Western blot analysis. Taken together, our findings indicate that C. japonicum extract induces the inhibition of MCF-7 cell growth at both the proliferation and apoptosis levels.
- Subjects :
- Cancer Research
Cell growth
Cell
food and beverages
Cell cycle
Biology
medicine.disease_cause
Biochemistry
Molecular biology
medicine.anatomical_structure
Oncology
MCF-7
Apoptosis
parasitic diseases
Cancer cell
Immunology
Genetics
medicine
Molecular Medicine
MTT assay
Carcinogenesis
Molecular Biology
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 3
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....25195e214a51e9e5f0936803289728ac