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Type 1 Diabetes: Urinary Proteomics and Protein Network Analysis Support Perturbation of Lysosomal Function
- Source :
- Theranostics
- Publication Year :
- 2017
- Publisher :
- Ivyspring International Publisher, 2017.
-
Abstract
- While insulin replacement therapy restores the health and prevents the onset of diabetic complications (DC) for many decades, some T1D patients have elevated hemoglobin A1c values suggesting poor glycemic control, a risk factor of DC. We surveyed the stool microbiome and urinary proteome of a cohort of 220 adolescents and children, half of which had lived with T1D for an average of 7 years and half of which were healthy siblings. Phylogenetic analysis of the 16S rRNA gene did not reveal significant differences in gut microbial alpha-diversity comparing the two cohorts. The urinary proteome of T1D patients revealed increased abundances of several lysosomal proteins that correlated with elevated HbA1c values. In silico protein network analysis linked such proteins to extracellular matrix components and the glycoprotein LRG1. LRG1 is a prominent inflammation and neovascularization biomarker. We hypothesize that these changes implicate aberrant glycation of macromolecules that alter lysosomal function and metabolism in renal tubular epithelial cells, cells that line part of the upper urinary tract.
- Subjects :
- Adult
Male
0301 basic medicine
medicine.medical_specialty
Adolescent
Proteome
medicine.medical_treatment
Urinary system
Medicine (miscellaneous)
Inflammation
Urine
Young Adult
03 medical and health sciences
Protein network
Glycation
Internal medicine
medicine
Humans
Prospective Studies
Protein Interaction Maps
Child
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Upper urinary tract
Gut microbiome
Type 1 diabetes
business.industry
Insulin
Proteins
medicine.disease
Lysosome
Gastrointestinal Microbiome
3. Good health
Diabetes Mellitus, Type 1
030104 developmental biology
Endocrinology
LRG1
Case-Control Studies
Child, Preschool
Type 1 Diabetes
Female
medicine.symptom
Lysosomes
business
Research Paper
Urinary proteome
Subjects
Details
- ISSN :
- 18387640
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Theranostics
- Accession number :
- edsair.doi.dedup.....25188b0c2d3577775c5bdfe0ccad71af