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Development of endomyocardial fibrosis model using a cell patterning technique: In vitro interaction of cell coculture of 3T3 fibroblasts and RL-14 cardiomyocytes
- Source :
- PLoS ONE, Vol 15, Iss 2, p e0229158 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Cardiac functions can be altered by changes in the microstructure of the heart, i.e., remodeling of the cardiac tissue, which may activate pathologies such as hypertrophy, dilation, or cardiac fibrosis. Cardiac fibrosis can develop due to an excessive deposition of extracellular matrix proteins, which are products of the activation of fibroblasts. In this context, the anatomical-histological change may interfere with the functioning of the cardiac tissue, which requires specialized cells for its operation. The purpose of the present study was to determine the cellular interactions and morphological changes in cocultures of 3T3 fibroblasts and RL-14 cardiomyocytes via the generation of a platform an in vitro model. For this purpose, a platform emulating the biological characteristics of endomyocardial fibrosis was generated using a cell patterning technique to study morphological cellular changes in compact and irregular patterns of fibrosis. It was found that cellular patterns emulating the geometrical distributions of endomyocardial fibrosis generated morphological changes after interaction of the RL-14 cardiomyocytes with the 3T3 fibroblasts. Through this study, it was possible to evaluate biological characteristics such as cell proliferation, adhesion, and spatial distribution, which are directly related to the type of emulated endomyocardial fibrosis. This research concluded that fibroblasts inhibited the proliferation of cardiomyocytes via their interaction with specific microarchitectures. This behavior is consistent with the histopathological distribution of cardiac fibrosis; therefore, the platform developed in this research could be useful for the in vitro assessment of cellular microdomains. This would allow for the experimental determination of interactions with drugs, substrates, or biomaterials within the engineering of cardiac tissues.
- Subjects :
- 0301 basic medicine
Cardiac fibrosis
Cellular differentiation
Cell Lines
Endomyocardial fibrosis
Cell Communication
030204 cardiovascular system & hematology
Biochemistry
Extracellular matrix
Mice
0302 clinical medicine
Fibrosis
Animal Cells
Medicine and Health Sciences
Myocyte
Myocytes, Cardiac
Connective Tissue Cells
Cardiomyocytes
Multidisciplinary
Chemistry
3T3 Cells
Endomyocardial Fibrosis
Cell biology
Connective Tissue
Cell Processes
Medicine
Biological Cultures
Cellular Types
Anatomy
Research Article
Cell signaling
Science
Muscle Tissue
Research and Analysis Methods
Models, Biological
Cell Line
03 medical and health sciences
Population Metrics
medicine
Cell Adhesion
Vimentin
Animals
Humans
Cell adhesion
Cell Proliferation
Population Density
Muscle Cells
Population Biology
Biology and Life Sciences
Proteins
Cell Biology
Fibroblasts
medicine.disease
Coculture Techniques
Cytoskeletal Proteins
030104 developmental biology
Biological Tissue
Cultured Fibroblasts
Collagens
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....250dbaec3365beea711d88a7478a2d98