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Cartilage, bone and synovial histomorphometry in animal models of osteoarthritis
- Source :
- Osteoarthritis and Cartilage. 18:S106-S112
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Summary Objective This review focuses on histomorphometry for assessing the pathological changes in various compartments of the joint including cartilage, bone and synovium in animal models of osteoarthritis (OA). Methods Different methodological approaches are presented concerning sampling, embedding, sectioning, staining, mounting of stained sections and measurement of histomorphometric parameters using automated and semi-automated methods. Notes are provided describing some methods in greater detail. Results Histomorphometry allows a significant gain of objectivity, accuracy and reproducibility in the quantification of the main histological parameters which best characterize OA in the affected joint (cartilage thickness (CT), chondrocyte size and density, cartilage fissure, proteoglycan (PG) content, subchondral bone plate thickness (SBPT), thickness of synovial living cell layer) in animal models. Conclusion Use of histomorphometry could contribute to a better quantification of histological differences between control and OA animals. Contributing also to the introduction of normative data, it is a major advantage for therapeutic assessments in experimental OA and particularly for the analytical comparison of the efficacy of disease modifying OA drugs (DMOAD).
- Subjects :
- Cartilage, Articular
Pathology
medicine.medical_specialty
Cartilage fissure
Histology
Biomedical Engineering
Living cell
Osteoarthritis
Chondrocyte
Image analysis
Rheumatology
Chondrocyte density and size
Proteoglycan content
Subchondral bone plate thickness
Synovium
Animals
Medicine
Orthopedics and Sports Medicine
Bone
Goldner’s trichrome
Histomorphometry
Histocytological Preparation Techniques
Cartilage thickness
business.industry
Cartilage
Synovial Membrane
medicine.disease
Arthritis, Experimental
Disease Models, Animal
medicine.anatomical_structure
Subchondral bone
Safranin O
Joints
Proteoglycans
business
Cartilage bone
Subjects
Details
- ISSN :
- 10634584
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Osteoarthritis and Cartilage
- Accession number :
- edsair.doi.dedup.....24f57f35a832f86ab48f150fe5441a64