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MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
- Source :
- Bioengineered, article-version (VoR) Version of Record, Bioengineered, Vol 12, Iss 2, Pp 10713-10722 (2021)
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- We study whether microRNA miR-30a inhibits the autophagy through transforming growth factor (TGF)-β/Smad4 to generate cisplatin (DDP) resistance in ovarian cancer cells. The expression of miR-30a, Smad4, and TGF-β was detected in the serum of ovarian cancer patients and DDP-resistant cell lines (A2780) by quantitative real-time polymerase chain reaction (qRT-PCR). Computational search and western blotting were used to demonstrate the downstream target of miR-30a in ovarian cancer cells. Cell viability was measured with CCK8 assay. Apoptosis and autophagy of ovarian cancer cells were analyzed by flow cytometry and transmission electron microscopy, and the expressions of Beclin1 and LC3II protein were detected by western blotting. Expression of miR-30a was significantly decreased, while expressions of TGF-β and Smad4 mRNA were increased in serum of ovarian cancer patients after DDP chemotherapy as well as in DDP-resistant cells. Activation of autophagy contributed to DDP-resistance cells. Moreover, Bioinformatics software predicted Smad4 to be a target of miR-30a. Overexpression of miR-30a decreased the expression of Smad4 and TGF-β. Additionally, miR-30a-overexpressing inhibited DDP-induce autophagy and promoted DDP-resistant cell apoptosis. In conclusion, miR-30a mediates DDP resistance in ovarian cancer by inhibiting autophagy via the TGF-β/Smad4 pathway.
- Subjects :
- endocrine system diseases
Down-Regulation
Apoptosis
Bioengineering
Applied Microbiology and Biotechnology
Sensitivity
Western blot
Ovarian cancer
Transforming Growth Factor beta
Cell Line, Tumor
microRNA
Autophagy
medicine
Humans
Viability assay
Aged
Smad4 Protein
Ovarian Neoplasms
Cisplatin
Base Sequence
medicine.diagnostic_test
Chemistry
General Medicine
Middle Aged
medicine.disease
Up-Regulation
Gene Expression Regulation, Neoplastic
MicroRNAs
Drug Resistance, Neoplasm
Cell culture
Cancer research
Female
miR-30a
TP248.13-248.65
Research Article
Research Paper
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 21655987 and 21655979
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....24da0d0686249bfb60b975fb4988f0c3