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Rat epileptic seizures evoked by BmK αIV and its possible mechanisms involved in sodium channels
- Source :
- Toxicology and Applied Pharmacology. 220:235-242
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- This study showed that rat unilateral intracerebroventricular injection of BmK alphaIV, a sodium channel modulator derived from scorpion Buthus martensi Karsch, induced clusters of spikes, epileptic discharges and convulsion-related behavioral changes. BmK alphaIV potently promoted the release of endogenous glutamate from rat cerebrocortical synaptosomes. In vitro examination of the effect of BmK alphaIV on intrasynaptosomal free calcium concentration [Ca(2+)](i) and sodium concentration [Na(+)](i) revealed that BmK alphaIV-evoked glutamate release from synaptosomes was associated with an increase in Ca(2+) and Na(+) influx. Moreover, BmK alphaIV-mediated glutamate release and ion influx was completely blocked by tetrodotoxin, a blocker of sodium channel. Together, these results suggest that the induction of BmK alphaIV-evoked epileptic seizures may be involved in the modulation of BmK alphaIV on tetrodotoxin-sensitive sodium channels located on the nerve terminal, which subsequently enhances the Ca(2+) influx to cause an increase of glutamate release. These findings may provide some insight regarding the mechanism of neuronal action of BmK alphaIV in the central nervous system for understanding epileptogenesis involved in sodium channels.
- Subjects :
- Male
Time Factors
Sodium
Glutamic Acid
Scorpion Venoms
chemistry.chemical_element
Tetrodotoxin
Calcium
Pharmacology
Toxicology
Epileptogenesis
Sodium Channels
Potassium Chloride
Rats, Sprague-Dawley
chemistry.chemical_compound
Animals
Fluorometry
Injections, Intraventricular
Cerebral Cortex
Synaptosome
Epilepsy
Dose-Response Relationship, Drug
Sodium channel
Glutamate receptor
Electroencephalography
Rats
Electrophysiology
chemistry
Anesthesia
Sodium Channel Blockers
Synaptosomes
Subjects
Details
- ISSN :
- 0041008X
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- Toxicology and Applied Pharmacology
- Accession number :
- edsair.doi.dedup.....24d71e84334a69d2f286483298e6f66d