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Airway inflammation in cadmium-exposed rats is associated with pulmonary oxidative stress and emphysema
- Source :
- Free Radical Research. 40:241-250
- Publication Year :
- 2006
- Publisher :
- Informa UK Limited, 2006.
-
Abstract
- The aim of this study was to test the hypothesis that pulmonary inflammation and emphysema induced by cadmium (Cd) inhalation are associated with pulmonary oxidative stress. Two groups of Sprague Dawley rats were used: one vehicle-exposed group undergoing inhalation of NaCl (0.9%, n = 24) and one Cd-exposed group undergoing inhalation of CdCl(2) (0.1%, n = 24). The animals in the vehicle-and Cd-exposed groups were divided into 4 subgroups (n = 6 per group), which underwent either a single exposure (D2) of 1H or repeated exposures 3 times/week for 1H for a period of 3 weeks (3W), 5 weeks (5W) or 5 weeks followed by 2 weeks without exposure (5W + 2). At sacrifice, the left lung was fixed for histomorphometric analysis (median inter-wall distance, MIWD), whilst bronchoalveolar lavage fluid (BALF) was collected from the right lung. Cytological analysis of BALF was performed and BALF was analysed for oxidant markers 8-iso-PGF(2a), uric acid (UA), reduced (AA) and oxidised ascorbic acid (DHA) and reduced (GSH) and oxidised glutathione (GSSG). Cd-exposure induced a significant increase of BALF macrophages and neutrophils. 8-iso-PGF(2a), UA, GSH and GSSG were significantly increased at D2. At 5W and 5W + 2, AA and GSH were significantly lower in Cd-exposed rats, indicating antioxidant depletion. MIWD significantly increased in all repeatedly Cd-exposed groups, suggesting development of pulmonary emphysema. 8-iso-PGF(2a) and UA were positively correlated with macrophage and neutrophil counts. GSH, GSSG and 8-iso-PGF(2a) were negatively correlated with MIWD, indicating that Cd-induced emphysema could be associated with pulmonary oxidative stress.
- Subjects :
- Male
medicine.medical_specialty
Pathology
Antioxidant
Neutrophils
medicine.medical_treatment
Ascorbic Acid
Dinoprost
medicine.disease_cause
Biochemistry
Antioxidants
Rats, Sprague-Dawley
Pulmonary Disease, Chronic Obstructive
chemistry.chemical_compound
Internal medicine
medicine
Animals
Lung
Inflammation
medicine.diagnostic_test
Inhalation
business.industry
Macrophages
General Medicine
Glutathione
respiratory system
Ascorbic acid
Rats
Uric Acid
Oxidative Stress
Bronchoalveolar lavage
Endocrinology
medicine.anatomical_structure
Pulmonary Emphysema
chemistry
Uric acid
business
Bronchoalveolar Lavage Fluid
Oxidation-Reduction
Oxidative stress
Cadmium
Subjects
Details
- ISSN :
- 10292470 and 10715762
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Free Radical Research
- Accession number :
- edsair.doi.dedup.....24d383fcaa79944b1fa656ae81faa409