Additional file 7: of Changes in H3K27ac following lipopolysaccharide stimulation of nasopharyngeal epithelial cells

RELA activation following LPS treatment and binding at super-enhancers. A: Detroit 562 cells were treated with LPS at 1 μg/mL or fresh medium (no treatment) for 2 h and nuclear protein were extracted to be used in NFkB p65 transcription factor assay. No binding sites (no BS), wild type NFkB binding sites (WT BS) or mutant NFkB binding sites (mutant BS) were added in solution into the wells to check for RELA specificity. B: The cells were treated similarly and RELA activation was investigated at different time points for an extended period of time. The two curves correspond to independent experiments (dup = duplicate). C: Distribution of the RELA signal in Control-only (grey) and LPS-only (orange) Super-enhancers identified from the H3K27ac ChIP-seq data. (PDF 318 kb)