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Dried blood spots collected on filter paper: an international resource for the diagnosis and genetic characterization of human immunodeficiency virus Type-1
- Source :
- Scopus-Elsevier, Memórias do Instituto Oswaldo Cruz, Volume: 91, Issue: 3, Pages: 351-358, Published: JUN 1996, Memórias do Instituto Oswaldo Cruz., Vol 91, Iss 3, Pp 351-358 (1996)
- Publication Year :
- 1996
- Publisher :
- FapUNIFESP (SciELO), 1996.
-
Abstract
- The collection of dried blood spots (DBS) on filter paper provides a powerful approach for the development of large-scale, population-based screening programs. DBS methods are particularly valuable in developing countries and isolated rural regions where resources are limited. Large numbers of field specimens can be economically collected and shipped to centralized reference laboratories for genetic and (or) serological analysis. Alternatively, the dried blood can be stored and used as an archival resource to rapidly establish the frequency and distribution of newly recognized mutations, confirm patient identity or track the origins and emergence of newly identified pathogens. In this report, we describe how PCR-based technologies are beginning to interface with international screening programmes for the diagnosis and genetic characterization of human immunodeficiency virus type 1 (HIV-1). In particular, we review recent progress using DBS specimens to resolve the HIV-1 infection status of neonates, monitor the genetic evolution of HIV-1 during early infancy and establish a sentinel surveillance system for the systematic monitoring of HIV-1 genetic variation in Asia.
- Subjects :
- Paper
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine
Resource (biology)
lcsh:RC955-962
diagnosis
Population
lcsh:QR1-502
Drug Resistance
Human immunodeficiency virus (HIV)
Biology
medicine.disease_cause
Polymerase Chain Reaction
lcsh:Microbiology
Genetic Evolution
Genetic variation
medicine
Screening programs
Humans
Amino Acid Sequence
Dried blood
education
DNA Primers
AIDS Vaccines
Acquired Immunodeficiency Syndrome
Blood Specimen Collection
dried blood
education.field_of_study
Base Sequence
Infant, Newborn
AIDS Serodiagnosis
Genetic Variation
Infant
vaccines
Early infancy
Virology
Mutation
genetic variation
HIV-1
Sequence Analysis
Zidovudine
Filtration
Subjects
Details
- ISSN :
- 00740276
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Memórias do Instituto Oswaldo Cruz
- Accession number :
- edsair.doi.dedup.....24c288d6c5df4820a60664abc1e5ef9c