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Production and characterization of a novel HPV anti-L2 monoclonal antibody panel
- Source :
- Virology. 524:106-113
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The major capsid protein of HPV, L1, assembles into pentamers that form a T = 7 icosahedral particle, but the location of the co-assembled minor capsid protein, L2, remains controversial. Several researchers have developed useful monoclonal antibodies targeting L2, but most react with linear epitopes toward the N-terminus. As a means to better define the virus capsid and better assess the localization and exposure of L2 epitopes in the context of assembled HPV, we have developed a panel of 30 monoclonal antibodies (mAbs) which target the N-terminus of L2 amino acids 11–200, previously defined as a broadly protective immunogen. Select mAbs were processed with enzymes and anti-L2 Fabs were generated. These new mAb/Fab probes will be beneficial in future studies to unravel the placement of L2 and to help better define the role of L2 in the HPV lifecycle and the nature of the broadly protective epitopes.
- Subjects :
- 0301 basic medicine
Immunogen
Future studies
medicine.drug_class
Context (language use)
Alphapapillomavirus
Cross Reactions
Antibodies, Viral
Monoclonal antibody
Article
Epitope
Epitopes
Mice
03 medical and health sciences
Virology
medicine
Animals
Humans
Mice, Inbred BALB C
biology
Papillomavirus Infections
Virion
Antibodies, Monoclonal
Oncogene Proteins, Viral
030104 developmental biology
Capsid
biology.protein
Capsid Proteins
Antibody
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 524
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....24c039170a954970c61c870fa4ab9207