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Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model
- Source :
- Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018), Scientific Reports
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Osteoporosis, characterized by bone mass reduction and increased fractures, has become a global health problem that seriously affects the health of people, especially postmenopausal women; however, the current pathogenesis of postmenopausal osteoporosis (PMOP) has not been thoroughly elucidated to date. In this study, bilateral ovariectomy was performed to establish an OVX mouse model of osteoporosis. UPLC-Q-TOF-MS-based lipidomics in combination with metabolomics were used to analyze the femur tissue of osteoporosis mice. We found that 11 polar metabolites and 93 lipid metabolites were significantly changed and were involved in amino acid metabolism, nucleotide metabolism and lipid metabolism. Among the lipids, fatty acyls, glycerolipids, glycerophospholipids, sphingolipids and sterols showed robust changes. These results revealed that several metabolic disorders caused by changes in the hormone levels in OVX, especially disordered lipid metabolism, are closely related to the imbalance between bone resorption and formation and may underlie the development of PMOP. The data generated via lipidomics and metabolomics presented in this study shows good applicability and wide coverage in the construction of the metabolic profile of bone tissue. Therefore, this approach may provide the pathway focusing and data support at the metabolite level for the in-depth mechanism of PMOP.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Science
Osteoporosis
Glycerophospholipids
Bone tissue
Bone and Bones
Article
Bone resorption
Postmenopausal Osteoporosis
Mice
03 medical and health sciences
Metabolomics
Internal medicine
Lipidomics
medicine
Metabolome
Animals
Humans
Femur
Osteoporosis, Postmenopausal
Multidisciplinary
business.industry
Computational Biology
Lipid metabolism
X-Ray Microtomography
Chromatography, Ion Exchange
Lipid Metabolism
medicine.disease
Sphingolipid
Disease Models, Animal
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Osteoporosis Mice
Bone Volume/total Volume (BV/TV)
Lipidomic Analysis
Medicine
Female
business
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....24b3f17908290396cc4aa3d538e0befa