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Bis induces growth inhibition and differentiation of HL-60 cells via up-regulation of p27
- Source :
- Experimental & Molecular Medicine. 37:624-630
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- Bis (Bag-3, CAIR), a Bcl-2-interacting protein, promotes the anti-apoptotic activity of Bcl-2 and increased levels of Bis have been observed in several disease models. The involvement of Bcl-2 and some Bcl-2-binding proteins in differentiation has recently been reported. However, the relevance of Bis to cellular differentiation remains unknown. The findings herein show that Bis expression is up-regulated during the differentiation of HL-60 cells. To investigate the effect of Bis expression on differentiation, we established Bis-overexpressing HL-60 cells (HL-60-bis). HL-60-bis cells have a low nuclear: cytoplasmic ratio and indented nucleus in Wright- Giemsa staining, and an increased expression of CD11b in immunofluorescence study, indicating the promotion of differentiation. The overexpression of Bis also resulted in a retarded cell growth rate, accompanied by the accumulation of HL-60 cells at the G0/G1 phase of the cell cycle, which was sustained during the differentiation process. Western blot analysis revealed that the expression of p27, a representative inducer of cell cycle arrest at the G1 phase, was increased 2.5-fold in HL-60-bis cells compared to HL-60-neo cells. These results suggest that the Bis induced growth inhibition of HL-60 cells promotes G0/G1 phase arrest via up-regulation of p27, which seems to be a prerequisite for differentiation. Further studies will be required to define the exact roles of Bis on cellular differentiation more precisely.
- Subjects :
- Cell cycle checkpoint
Cellular differentiation
Clinical Biochemistry
Gene Expression
HL-60 Cells
Biochemistry
chemistry.chemical_compound
Downregulation and upregulation
Western blot
medicine
Humans
Cell Shape
Molecular Biology
Cells, Cultured
Adaptor Proteins, Signal Transducing
Cell Proliferation
biology
medicine.diagnostic_test
Cell Differentiation
Cell cycle
Flow Cytometry
Molecular biology
Up-Regulation
Cell biology
Integrin alpha M
chemistry
Cytoplasm
biology.protein
Molecular Medicine
Growth inhibition
Apoptosis Regulatory Proteins
Carrier Proteins
Cyclin-Dependent Kinase Inhibitor p27
Subjects
Details
- ISSN :
- 20926413
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Experimental & Molecular Medicine
- Accession number :
- edsair.doi.dedup.....24ae4bdafadfd9ee4143e70bcbab97ca