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Wnt5a Suppresses β-catenin Signaling during Hair Follicle Regeneration
- Source :
- International Journal of Medical Sciences
- Publication Year :
- 2016
- Publisher :
- Ivyspring International Publisher, 2016.
-
Abstract
- Hair follicles display periodic growth. Wnt signaling is a critical regulator for hair follicle regeneration. Previously, we reported that Wnt5a inhibits the telogen-to-anagen transition of hair follicles, but the mechanism by which this process occurs has not yet been reported. Here, we determined the expression patterns of Wnt signaling pathway molecules by quantitative reverse transcription polymerase chain reaction, western blot, and immunohistochemistry and found that β-catenin signaling was suppressed by Wnt5a. We then compared the phenotypes and expression patterns following β-catenin knockdown and Wnt5a overexpression during hair follicle regeneration induced by hair depilation and observed similar patterns. In addition, we performed a rescue experiment in the JB6 cell line and found that the inhibitory effect of Wnt5a on cell proliferation could be rescued by the addition of Wnt3a. Our data reveal that Wnt5a suppresses the activation of β-catenin signaling during hair follicle regeneration.
- Subjects :
- 0301 basic medicine
Beta-catenin
Wnt-5a Protein
Cell Line
Wnt signaling pathway
regeneration
03 medical and health sciences
Wnt3A Protein
medicine
Humans
Regeneration
beta Catenin
Cell Proliferation
hair follicle
integumentary system
biology
Cell growth
Regeneration (biology)
Gene Expression Regulation, Developmental
General Medicine
β-catenin
Wnt5a
Hair follicle
Molecular biology
body regions
Reverse transcription polymerase chain reaction
030104 developmental biology
medicine.anatomical_structure
embryonic structures
biology.protein
sense organs
WNT3A
Research Paper
Subjects
Details
- ISSN :
- 14491907
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- International Journal of Medical Sciences
- Accession number :
- edsair.doi.dedup.....24a8695d17b4af76dd94267e4203d59d