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QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
- Source :
- PLoS ONE, Vol 11, Iss 3, p e0152795 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Low temperature is the primary factor to affect maize sowing in early spring. It is, therefore, vital for maize breeding programs to improve tolerance to low temperatures at seed germination stage. However, little is known about maize QTL involved in low-temperature germination ability. 243 lines of the intermated B73×Mo17 (IBM) Syn4 recombinant inbred line (RIL) population was used for QTL analysis of low-temperature germination ability. There were significant differences in germination-related traits under both conditions of low temperature (12°C/16 h, 18°C/8 h) and optimum temperature (28°C/24 h) between the parental lines. Only three QTL were identified for controlling optimum-temperature germination rate. Six QTL controlling low-temperature germination rate were detected on chromosome 4, 5, 6, 7 and 9, and contribution rate of single QTL explained between 3.39%~11.29%. In addition, six QTL controlling low-temperature primary root length were detected in chromosome 4, 5, 6, and 9, and the contribution rate of single QTL explained between 3.96%~8.41%. Four pairs of QTL were located at the same chromosome position and together controlled germination rate and primary root length under low temperature condition. The nearest markers apart from the corresponding QTL (only 0.01 cM) were umc1303 (265.1 cM) on chromosome 4, umc1 (246.4 cM) on chromosome 5, umc62 (459.1 cM) on chromosome 6, bnl14.28a (477.4 cM) on chromosome 9, respectively. A total of 3155 candidate genes were extracted from nine separate intervals based on the Maize Genetics and Genomics Database (http://www.maizegdb.org). Five candidate genes were selected for analysis as candidates putatively affecting seed germination and seedling growth at low temperature. The results provided a basis for further fine mapping, molecular marker assisted breeding and functional study of cold-tolerance at the stage of seed germination in maize.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Acclimatization
lcsh:Medicine
Plant Science
Plant Reproduction
01 natural sciences
Seed Germination
lcsh:Science
education.field_of_study
Multidisciplinary
biology
Chromosome Biology
Plant Anatomy
Chromosome Mapping
food and beverages
Agriculture
Plants
Cold Temperature
Phenotype
Germination
Plant Physiology
Seeds
Research Article
Quantitative Trait Loci
Population
Crops
Chromosome 9
Quantitative trait locus
Research and Analysis Methods
Zea mays
Chromosomes
Chromosomes, Plant
03 medical and health sciences
Model Organisms
Plant and Algal Models
Genetics
Grasses
Plant breeding
Molecular Biology Techniques
Linkage Mapping
education
Molecular Biology
Gene Mapping
lcsh:R
Organisms
Biology and Life Sciences
Sowing
Cell Biology
Chromosome Pairs
Chromosome 5
biology.organism_classification
Maize
Chromosome 4
Plant Breeding
030104 developmental biology
Agronomy
Genetic Loci
Seedlings
Seedling
lcsh:Q
Crop Science
Cereal Crops
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....249e858658bea3400bda0412970b8bb9
- Full Text :
- https://doi.org/10.1371/journal.pone.0152795