Back to Search
Start Over
Development of a miRNA surface-enhanced Raman scattering assay using benchtop and handheld Raman systems
- Source :
- Journal of Biomedical Optics. 23:1
- Publication Year :
- 2018
- Publisher :
- SPIE-Intl Soc Optical Eng, 2018.
-
Abstract
- DNA-functionalized nanoparticles, when paired with surface-enhanced Raman spectroscopy (SERS), can rapidly detect microRNA. However, widespread use of this approach is hindered by drawbacks associated with large and expensive benchtop Raman microscopes. MicroRNA-17 (miRNA-17) has emerged as a potential epigenetic indicator of preeclampsia, a condition that occurs during pregnancy. Biomarker detection using an SERS point-of-care device could enable prompt diagnosis and prevention as early as the first trimester. Recently, strides have been made in developing portable Raman systems for field applications. An SERS assay for miRNA-17 was assessed and translated from traditional benchtop Raman microscopes to a handheld system. Three different photoactive molecules were compared as potential Raman reporter molecules: a chromophore, malachite green isothiocyanate (MGITC), a fluorophore, tetramethylrhodamine isothiocyanate, and a polarizable small molecule 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB). For the benchtop Raman microscope, the DTNB-labeled assay yielded the greatest sensitivity under 532-nm laser excitation, but the MGITC-labeled assay prevailed at 785 nm. Conversely, DTNB was preferable for the miniaturized 785-nm Raman system. This comparison showed significant SERS enhancement variation in response to 1-nM miRNA-17, implying that the sensitivity of the assay may be more heavily dependent on the excitation wavelength, instrumentation, and Raman reporter chosen than on the plasmonic coupling from DNA/miRNA-mediated nanoparticle assemblies.
- Subjects :
- Materials science
Microscope
Fluorophore
Biomedical Engineering
Nanotechnology
02 engineering and technology
Spectrum Analysis, Raman
010402 general chemistry
01 natural sciences
law.invention
Biomaterials
symbols.namesake
chemistry.chemical_compound
Isothiocyanates
law
Rosaniline Dyes
Coloring Agents
Plasmon
DNA
Surface-enhanced Raman spectroscopy
021001 nanoscience & nanotechnology
Small molecule
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Electronic, Optical and Magnetic Materials
MicroRNAs
chemistry
Point-of-Care Testing
symbols
Nanoparticles
Raman microscope
0210 nano-technology
Raman spectroscopy
Raman scattering
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....249c1cf4995d4bd7d91ccfb46f649722