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In Silico Analysis of ACE Inhibitory Peptides from Chloroplast Proteins of Red Alga Grateloupia asiatica
- Source :
- Marine Biotechnology. 22:391-402
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Inhibition of angiotensin I-converting enzyme (ACE) is one of the key factors to repress high blood pressure. Although many studies have been reported that seaweed protein hydrolysates showed the ACE inhibitory activity, the comprehensive understanding of the relationship was still unclear. In this study, we employed chloroplast genome for in silico analysis and compared it with in vitro experiments. We first extracted water-soluble proteins (WSP) from red alga Grateloupia asiatica, which contained mainly PE, PC, APC, and Rbc, and prepared WSP hydrolysate by thermolysin, resulting that the hydrolysate showed ACE inhibitory activity. Then, we determined the complete chloroplast genome of G. asiatica (187,518 bp: 206 protein-coding genes, 29 tRNA, and 3 rRNA) and clarified the amino acid sequences of main WSP, i.e., phycobiliproteins and Rubisco, to perform in silico analysis. Consequently, 190 potential ACE inhibitory peptides existed in the main WSP sequences, and 21 peptides were obtained by in silico thermolysin digestion. By comparing in vitro and in silico analyses, in vitro ACE inhibitory activity was correlated to the IC50 value from in silico digestion. Therefore, in silico approach provides insight into the comprehensive understanding of the potential bioactive peptides from seaweed proteins.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chloroplasts
ACE inhibitory peptide
In silico
Angiotensin-Converting Enzyme Inhibitors
In silico analysis
Biology
01 natural sciences
Applied Microbiology and Biotechnology
Hydrolysate
Chloroplast Proteins
03 medical and health sciences
Thermolysin
010608 biotechnology
Computer Simulation
Chloroplast genome
Amino Acid Sequence
chemistry.chemical_classification
Red alga
Algal Proteins
RuBisCO
Grateloupia asiatica
Amino acid
Chloroplast
030104 developmental biology
Enzyme
chemistry
Biochemistry
Rhodophyta
biology.protein
Subjects
Details
- ISSN :
- 14362236 and 14362228
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Marine Biotechnology
- Accession number :
- edsair.doi.dedup.....249af6fbdf30358b73a8c2e5835deba2