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C57BL/6 and 129/Sv mice: genetic difference to renal ischemia-reperfusion
- Source :
- Journal of nephrology. 25(5)
- Publication Year :
- 2011
-
Abstract
- Background: C57BL/6 and 129/Sv are the 2 most com- monly used strains of mice in renal ischemia-reper- fusion injury (IRI) studies, yet there are currently no studies that contrast differences in the degree of renal injury after ischemia-reperfusion. Methods: To evaluate renal IRI in male C57BL/6 and 129/Sv mice, we performed unilateral clamping of the left renal pedicle for 45 minutes and compared the de- gree of renal tissue damage and function. To measure function and tissue damage we examined: glomerular filtration rate (GFR; by inulin clearance), renal blood flow (RBF; by p-aminohippurate (PAH) clearance), renal morphology, immunohistochemistry for infiltrating leu- kocytes, and fibrogenic markers by Sirius red staining. Results: After unilateral IRI, 129/sv mice had signifi- cantly less GFR and RBF disfunction at both day 14 (d14) and d28. 129/sv mice also had significantly less acute tubular necrosis on d1 and fewer infiltrating leu- kocytes on d28, as well as less collagen deposition on d28 than C57BL/6 mice. Conclusions: C57BL/6 mice were much more sensitive to damage caused by renal IRI than are 129/Sv mice.
- Subjects :
- Male
medicine.medical_specialty
Mice, 129 Strain
Time Factors
Biopsy
Urology
Renal function
urologic and male genital diseases
Kidney
Severity of Illness Index
Collagen Type I
Monocytes
Renal Circulation
chemistry.chemical_compound
Mice
Species Specificity
medicine
Animals
Genetic Predisposition to Disease
Lymphocytes
Sirius Red
Acute tubular necrosis
Renal circulation
medicine.diagnostic_test
Staining and Labeling
urogenital system
business.industry
Macrophages
Kidney metabolism
medicine.disease
Immunohistochemistry
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Collagen Type III
Phenotype
chemistry
Nephrology
Renal blood flow
Reperfusion Injury
Immunology
business
Biomarkers
Glomerular Filtration Rate
Subjects
Details
- ISSN :
- 17246059
- Volume :
- 25
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of nephrology
- Accession number :
- edsair.doi.dedup.....2499788b72e43c38d03788443851a371