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Genetic restriction of murine hepatitis virus type 3 expression in liver and brain: comparative study in BALB/c and C3H mice by immunochemistry and hybridization in situ
- Source :
- Archives of Virology
- Publication Year :
- 1993
- Publisher :
- Springer-Verlag, 1993.
-
Abstract
- Summary To study the host-dependent genetic variations in murine hepatitis virus type 3 (MHV 3) induced diseases, we localized the sites of MHV 3 (Mill Hill strain) expression within liver and brain by immunohistochemistry or hybridization in situ. Two strains of mice were studied: BALB/c mice, which develop an acute and lethal hepatitis and C3H mice which develop a chronic brain infection. In BALB/c mice, viral RNA and antigens appeared during the first 24 h post infection (p.i.) in liver, whereas viral RNA was barely detectable in brain, up until death at day 3 p.i. In C3H mice, viral RNA and antigens were detected simultaneously in liver and brain only at day 2 p.i. In brain, the virus was detected in meningeal and ependymal cells and in perivascular cortical areas (days 5 and 7 p.i.). After day 49, the virus was no longer detected in brain parenchyma, but persisted in meningeal cells. Two host-dependent genetic differences in viral processing were observed in the liver: (1) the virus was first detected in Kupffer cells in BALB/c mice and mostly in hepatocytes in C3H mice; (2) in BALB/c mice, the 180 kDa S viral glycoprotein appeared more frequently cleaved in 90 kDa form than in C3H mice.
- Subjects :
- Coronaviridae Infections
viruses
Genetic Restriction
In situ hybridization
Biology
medicine.disease_cause
Virus
BALB/c
Hepatitis
Cortical Area
Immunoenzyme Techniques
Mice
Species Specificity
Virology
Parenchyma
Immunochemistry
medicine
Coronaviridae
Animals
Genetic Predisposition to Disease
In Situ Hybridization
Coronavirus
Mice, Inbred BALB C
Mice, Inbred C3H
Murine hepatitis virus
Brain
General Medicine
biology.organism_classification
medicine.disease
Original Papers
Liver
Hepatitis, Viral, Animal
Kupffer Cell
Genetic Difference
Subjects
Details
- Language :
- English
- ISSN :
- 14328798 and 03048608
- Volume :
- 130
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....2498d98c7c0fa24882836a0ee18cd2be