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Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan
- Source :
- Journal of Microbiology, Immunology and Infection, Vol 50, Iss 5, Pp 640-645 (2017)
- Publication Year :
- 2017
- Publisher :
- Elsevier, 2017.
-
Abstract
- Background/Purpose The purpose of this study is to investigate the predominant clones of carbapenem-resistant Acinetobacter baumannii (CRAB) in our hospital in Taiwan by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) technique. Methods We collected 108 non-duplicate A. baumannii clinical blood isolates from January 2012 to December 2013 in MacKay Memorial Hospital. PFGE and MLST were used for typing the A. baumannii isolates and for investigation of the predominant clones. Bacteria isolates were screened by polymerase chain reaction for the presence of the carbapenemase-encoding genes. Results All 108 isolates were classified as 33 pulsotypes by PFGE. The predominant clones were pulsotype 10 (12.04%) in 2012 and pulsotype 8 (16.67%) in 2013, respectively. The 31 predominant pulsotype isolates were typed by MLST, and ST787 (54.84%) and ST455 (45.16%) were identified. All isolates carried bla OXA-51-like genes, and bla OXA-23-like genes was founded in 101 isolates (93.52%). Other identified resistance genes included bla OXA-24-like and bla OXA-IMP . Conclusion To the best of our knowledge, this study is the first to describe the microbiological characteristics of CRAB ST787, which carried high genetic resistance to carbapenem, but remained susceptible to colistin. CRAB ST787 was the predominant clone in our hospital in the study period.
- Subjects :
- 0301 basic medicine
Acinetobacter baumannii
Carbapenemase gene
Carbapenem
lcsh:QR1-502
Polymerase Chain Reaction
lcsh:Microbiology
law.invention
Tertiary Care Centers
law
polycyclic compounds
Immunology and Allergy
Polymerase chain reaction
biology
Pulsotype
General Medicine
PFGE
Anti-Bacterial Agents
Electrophoresis, Gel, Pulsed-Field
Infectious Diseases
Acinetobacter Infections
medicine.drug
MLST
Microbiology (medical)
Sequence type
030106 microbiology
Taiwan
Microbial Sensitivity Tests
beta-Lactamases
Teaching hospital
Microbiology
03 medical and health sciences
Bacterial Proteins
Drug Resistance, Bacterial
medicine
Pulsed-field gel electrophoresis
Humans
Typing
Hospitals, Teaching
General Immunology and Microbiology
Colistin
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
bacterial infections and mycoses
Carbapenems
Multilocus sequence typing
bacteria
Multilocus Sequence Typing
Subjects
Details
- Language :
- English
- ISSN :
- 16841182
- Volume :
- 50
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology, Immunology and Infection
- Accession number :
- edsair.doi.dedup.....249476687c0d14ebcb12f13fe2d8aab0