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In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber
- Source :
- Molecules, Volume 25, Issue 21
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- This study measured the proliferative activity of malto-oligosaccharide (MOS) as a prebiotic against Bifidobacteria, resistance to digestion in vitro, and changes during in vitro fermentation by human fecal microorganisms. It consisted of 21.74%, 18.84%, and 11.76% of maltotriose, maltotetraose, and maltopentaose produced by amylase (HATT), respectively. When 1% of MOS was added to a modified PYF medium as the carbon source, proliferation of Bifidobacterium breve was increased significantly. During the in vitro digestion test, MOS was partially degraded by intestinal enzymes. Fermentation characteristics by human fecal microorganisms were evaluated by adding 1% galacto-oligosaccharide (GOS), as well as 1% and 2% MOS as carbon sources to the basal medium, respectively. In comparison with the addition of 1% of MOS and GOS, the total short chain fatty acid (SCFA) content increased over time when 2% of MOS was added. The species diversity and richness of intestinal microbiota increased significantly with 2% MOS compared to those with 1% GOS. In addition, the 2% addition of MOS reduced intestinal pathobiont microorganisms and increased commensal microorganisms including Bifidobacterium genus. Collectively, MOS produced by amylase increased the SCFA production and enhanced the growth of beneficial bacteria during in vitro fermentation by human fecal microbiota.
- Subjects :
- Dietary Fiber
Male
0301 basic medicine
in vitro fermentation
medicine.medical_treatment
ved/biology.organism_classification_rank.species
Oligosaccharides
Pharmaceutical Science
Analytical Chemistry
Feces
chemistry.chemical_compound
malto-oligosaccharide
fluids and secretions
Drug Discovery
Anaerobiosis
Food science
Amylase
reproductive and urinary physiology
Bifidobacterium
Bifidobacterium breve
biology
Chemistry
Short-chain fatty acid
Chemistry (miscellaneous)
Amylases
embryonic structures
Molecular Medicine
Digestion
Adult
musculoskeletal diseases
Article
Young Adult
03 medical and health sciences
medicine
Maltotriose
Humans
Physical and Theoretical Chemistry
Maltose
Cell Proliferation
030109 nutrition & dietetics
ved/biology
urogenital system
Prebiotic
Organic Chemistry
Galactose
Water
Fatty Acids, Volatile
biology.organism_classification
SCFA
Carbon
Gastrointestinal Microbiome
030104 developmental biology
Fermentation
biology.protein
prebiotics
Trisaccharides
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....2492d715fe4815a644a384ef8b797435
- Full Text :
- https://doi.org/10.3390/molecules25215201